{"product_id":"itga4-antibody-integrin-alpha-4-bha17135696","title":"ITGA4 Antibody \/ Integrin Alpha 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eITGA4 Antibody \/ Integrin Alpha 4 is a anti-ITGA4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ITGA4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIntegrin alpha 4 antibody detects Integrin alpha-4 (encoded by ITGA4), a cell surface adhesion receptor that mediates interactions between cells and the extracellular matrix, primarily through binding to vascular cell adhesion molecule 1 (VCAM1) and fibronectin. Encoded by the ITGA4 gene on chromosome 2q31.3, this protein pairs with the beta-1 (ITGB1) or beta-7 (ITGB7) subunit to form functional integrin heterodimers such as alpha4beta1 (VLA-4) and alpha4beta7. These complexes regulate leukocyte adhesion, migration, and immune cell trafficking across vascular and epithelial barriers.\u003cbr\u003e\u003cbr\u003eIntegrin alpha-4 is a transmembrane glycoprotein composed of a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail that links to cytoskeletal and signaling molecules. It facilitates rolling and firm adhesion of lymphocytes and monocytes during inflammatory responses, guiding immune cells to sites of tissue injury or infection. In the nervous system, it contributes to axonal guidance and glial cell adhesion.\u003cbr\u003e\u003cbr\u003eThe ITGA4 antibody is widely used in immunology, oncology, and vascular biology research to study adhesion, immune cell migration, and integrin-mediated signaling. Flow cytometry and western blot analyses identify Integrin alpha-4 as a 150 kilodalton band, while immunohistochemistry reveals membrane staining on leukocytes, endothelium, and fibroblasts. This antibody provides a means to examine integrin expression dynamics and cell adhesion regulation under physiological and pathological conditions.\u003cbr\u003e\u003cbr\u003eIntegrin alpha-4 is clinically relevant as a therapeutic target in multiple sclerosis and Crohn's disease, where monoclonal antibodies blocking alpha4-integrin (e.g., natalizumab) reduce leukocyte infiltration and inflammation. Aberrant expression also contributes to cancer metastasis and fibrotic diseases by enhancing cell adhesion and migration. The ITGA4 antibody supports investigations into these mechanisms by detecting endogenous protein in tissues and cell lines.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ITGA4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ITGA4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ITGA4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ITGA4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ITGA4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047301833069,"sku":"FY12794","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_aca7f7a1-d01d-487d-9042-9c694dfcf53a.jpg?v=1782237039","url":"https:\/\/www.ebiohippo.com\/products\/itga4-antibody-integrin-alpha-4-bha17135696","provider":"BioHippo","version":"1.0","type":"link"}