{"product_id":"itga9-antibody-integrin-alpha-9-bha17135589","title":"ITGA9 Antibody \/ Integrin alpha 9","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eITGA9 Antibody \/ Integrin alpha 9 is a anti-ITGA9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ITGA9\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eITGA9 antibody detects Integrin alpha-9, a transmembrane receptor that mediates cell adhesion, migration, and signal transduction by binding to extracellular matrix (ECM) ligands such as tenascin-C, osteopontin, and VCAM1. Integrins are heterodimeric proteins composed of alpha and beta subunits; Integrin alpha-9 pairs with the beta-1 subunit to form the alpha9beta1 integrin complex. This receptor is involved in developmental morphogenesis, angiogenesis, epithelial integrity, and immune cell trafficking. The alpha9beta1 integrin recognizes diverse ligands containing the LDV or SVVYGLR motifs and promotes cytoskeletal reorganization and cell movement through activation of focal adhesion kinase and Rho-family GTPases.\u003cbr\u003e\u003cbr\u003eIntegrin alpha-9 is encoded by the ITGA9 gene located on chromosome 3p22.2. It is expressed in smooth muscle cells, fibroblasts, airway epithelium, and leukocytes. During embryonic development, Integrin alpha-9 contributes to lymphatic valve formation and respiratory system maturation. Loss of ITGA9 function in animal models leads to perinatal lethality due to chylothorax, underscoring its essential role in lymphatic and pulmonary development. In adult tissues, Integrin alpha-9 supports wound healing and inflammation by mediating adhesion of leukocytes to endothelial and epithelial cells.\u003cbr\u003e\u003cbr\u003eThe ITGA9 antibody is used in cell biology, cancer, and immunology research to study integrin-mediated adhesion and signaling. In immunohistochemistry, it detects membrane-associated staining in epithelial and stromal cells. Western blot analysis identifies a ~130 kilodalton band corresponding to the mature glycosylated protein. Integrin alpha-9 participates in epithelial-mesenchymal transition and tumor metastasis, where increased expression correlates with invasive behavior. Blocking ITGA9 function has been shown to suppress cancer cell migration and angiogenesis, highlighting its potential as a therapeutic target.\u003cbr\u003e\u003cbr\u003eIntegrin alpha-9 interacts with cytoplasmic partners such as talin and kindlin to transmit bidirectional signaling between the ECM and intracellular environment. Activation of this receptor regulates cellular responses including proliferation, differentiation, and immune recruitment. The ITGA9 antibody enables detailed analysis of these signaling events and provides insight into how integrin networks integrate mechanical and chemical cues during tissue remodeling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ITGA9 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ITGA9 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ITGA9 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ITGA9 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ITGA9+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296065901,"sku":"FY12686","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6ab760bb-c641-4533-93de-3571701e3743.jpg?v=1782237025","url":"https:\/\/www.ebiohippo.com\/products\/itga9-antibody-integrin-alpha-9-bha17135589","provider":"BioHippo","version":"1.0","type":"link"}