{"product_id":"itpkc-antibody-inositol-trisphosphate-3-kinase-c-bha17135528","title":"ITPKC Antibody \/ Inositol-trisphosphate 3-kinase C","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eITPKC Antibody \/ Inositol-trisphosphate 3-kinase C is a anti-ITPKC Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ITPKC\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eITPKC antibody detects Inositol-trisphosphate 3-kinase C, a signaling enzyme that phosphorylates inositol 1,4,5-trisphosphate (IP3) to generate inositol 1,3,4,5-tetrakisphosphate (IP4). ITPKC serves as a critical regulator of intracellular calcium signaling, immune activation, and vascular inflammation. The ITPKC antibody is widely used in immunology, signal transduction, and cardiovascular research to study calcium homeostasis and immune cell regulation.\u003cbr\u003e\u003cbr\u003eITPKC is encoded by the ITPKC gene located on human chromosome 19q13.2. The protein is approximately 461 amino acids long and localizes to the cytoplasm, where it modulates calcium flux by regulating IP3 metabolism. Through phosphorylation of IP3, ITPKC reduces calcium release from the endoplasmic reticulum, thereby attenuating NFAT activation and T-cell proliferation.\u003cbr\u003e\u003cbr\u003eThe ITPKC antibody detects a 52 kilodalton band by western blot and exhibits diffuse cytoplasmic staining under fluorescence microscopy. ITPKC plays a pivotal role in immune regulation, particularly in T-cell receptor signaling. Genetic variants of ITPKC are associated with Kawasaki disease, an inflammatory vascular disorder, due to dysregulated calcium-dependent immune activation.\u003cbr\u003e\u003cbr\u003eBeyond immunity, ITPKC influences endothelial barrier function and smooth muscle contraction, linking it to cardiovascular homeostasis. It also interacts with other kinases and phosphatases in the phosphoinositide signaling pathway, integrating metabolic and signaling cues. In cancer cells, ITPKC expression affects proliferation and angiogenesis by controlling intracellular calcium oscillations.\u003cbr\u003e\u003cbr\u003eBecause of its dual role in immune and vascular signaling, ITPKC is a key enzyme at the crossroads of inflammation and calcium dynamics.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ITPKC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ITPKC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ITPKC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ITPKC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ITPKC+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047295213933,"sku":"FY12625","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_089451b5-9855-4e09-87fa-0363e2fb422d.jpg?v=1782237043","url":"https:\/\/www.ebiohippo.com\/products\/itpkc-antibody-inositol-trisphosphate-3-kinase-c-bha17135528","provider":"BioHippo","version":"1.0","type":"link"}