{"product_id":"k27-linkage-specific-ubiquitin-antibody-ubb-ubc-bha17136029","title":"K27-linkage Specific Ubiquitin Antibody \/ UBB \/ UBC","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eK27-linkage Specific Ubiquitin Antibody \/ UBB \/ UBC is a anti-K27-LINKAGE Rabbit antibody Recombinant Rabbit Monoclonal clone 31U80 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e K27-LINKAGE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31U80, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eK27-linkage specific ubiquitin antibody detects ubiquitin chains linked through lysine 27, generated from ubiquitin encoded by the UBB and UBC genes. Ubiquitin is a small regulatory protein that is covalently attached to substrate proteins, marking them for degradation or altering their function. Ubiquitin chains can be linked through different lysines, with each linkage type conferring distinct signaling functions. K27-linkage specific ubiquitin antibody provides researchers with a highly selective reagent to study lysine 27 ubiquitination and its roles in signaling, DNA repair, and immunity.\u003cbr\u003e\u003cbr\u003eUnlike the well characterized K48 and K63 chains, lysine 27 linked ubiquitin chains are less abundant but play specialized roles. Research using K27-linkage specific ubiquitin antibody has shown that these chains regulate mitochondrial homeostasis, DNA damage repair, and innate immune signaling. By targeting proteins for specific fates, K27 chains contribute to non-proteolytic ubiquitin functions, highlighting the complexity of the ubiquitin code.\u003cbr\u003e\u003cbr\u003eIn innate immunity, lysine 27 chains are involved in regulating antiviral signaling pathways such as RIG-I and MAVS. Studies with K27-linkage specific ubiquitin antibody have revealed that these chains promote activation of signaling complexes leading to interferon production. In DNA repair, K27 ubiquitin regulates assembly of repair factors at sites of damage. Dysregulation of K27 chains is linked to impaired genome stability and susceptibility to cancer.\u003cbr\u003e\u003cbr\u003eBecause ubiquitin modifications are dynamic and context dependent, linkage-specific antibodies are essential for dissecting the ubiquitin code. K27-linkage specific ubiquitin antibody allows researchers to distinguish lysine 27 linkages from other ubiquitin chains, enabling studies of how different linkages integrate into signaling networks. This specificity is crucial for understanding how ubiquitination encodes information beyond protein degradation.\u003cbr\u003e\u003cbr\u003eK27-linkage specific ubiquitin antibody is applied in western blotting, immunoprecipitation, and immunofluorescence. Western blotting detects K27-linked chains in cellular extracts, immunoprecipitation isolates proteins modified with this linkage, and immunofluorescence reveals dynamic localization after stress or immune stimulation. These methods highlight its value in ubiquitin signaling research.\u003cbr\u003e\u003cbr\u003eBy supplying validated K27-linkage specific ubiquitin antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=K27-LINKAGE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=K27-LINKAGE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=K27-LINKAGE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/K27-LINKAGE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=K27-LINKAGE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047313006957,"sku":"FY13127","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e1c5ea28-4c47-4079-a4ea-a4b4700d0c3f.jpg?v=1772019396","url":"https:\/\/www.ebiohippo.com\/products\/k27-linkage-specific-ubiquitin-antibody-ubb-ubc-bha17136029","provider":"BioHippo","version":"1.0","type":"link"}