{"product_id":"ki-67-antibody-mki67-bha17135866","title":"Ki-67 Antibody \/ MKI67","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eKi-67 Antibody \/ MKI67 is a anti-KI-67 Rabbit antibody Recombinant Rabbit Monoclonal clone 31K02 supplied in Liquid format. Recommended for workflows such as Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e KI-67\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31K02, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC, IF, ICC\/IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eKi-67 antibody detects the protein Ki-67, encoded by the MKI67 gene. Ki-67 is a nuclear protein expressed in proliferating cells and is one of the most widely used markers for cellular proliferation in research and clinical diagnostics. This protein is absent in resting cells (G0) but present during all active phases of the cell cycle, including G1, S, G2, and mitosis. Ki-67 antibody provides researchers with an essential tool to measure cell proliferation rates, assess tumor aggressiveness, and evaluate treatment responses in cancer studies. Its widespread use in histopathology reflects its reliability as a biomarker for growth activity.\u003cbr\u003e\u003cbr\u003eKi-67 is involved in ribosomal RNA transcription and organization of the nucleolus during interphase. During mitosis, it localizes to the perichromosomal layer, forming a structural scaffold that helps protect chromosomes and maintain proper segregation. Studies using Ki-67 antibody have revealed that depletion of this protein disrupts chromosomal condensation and segregation, underscoring its functional roles beyond serving as a proliferation marker. These insights have expanded its importance from being a passive marker of division to an active participant in cell cycle regulation.\u003cbr\u003e\u003cbr\u003eIn cancer biology, Ki-67 is one of the most important clinical biomarkers. Tumor samples are routinely stained with Ki-67 antibody to determine the proliferation index, which correlates with tumor grade, aggressiveness, and prognosis. High Ki-67 labeling indices are observed in fast-growing tumors such as breast, prostate, and brain cancers, and are associated with poor outcomes. Conversely, lower Ki-67 indices are linked to slower-growing neoplasms and better survival rates. Pathologists use Ki-67 antibody immunohistochemistry to quantify nuclear staining percentages in tumor biopsies, providing valuable prognostic information.\u003cbr\u003e\u003cbr\u003eBeyond oncology, Ki-67 antibody is applied in regenerative biology and developmental studies. Since this protein marks actively dividing cells, it is used to track stem cell proliferation, tissue regeneration, and embryonic development. Immunofluorescence assays with Ki-67 antibody help identify proliferative niches in tissues such as bone marrow, gut, and brain. Flow cytometry can also measure Ki-67 expression at the single-cell level, making it useful in studies of immune activation, hematopoietic stem cell dynamics, and vaccine responses.\u003cbr\u003e\u003cbr\u003eBy supplying validated Ki-67 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=KI-67 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=KI-67 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=KI-67 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/KI-67 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=KI-67+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047307927917,"sku":"FY12964","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ec6bdf6a-d2fe-49ba-b415-8994cb5d0a8d.jpg?v=1782237073","url":"https:\/\/www.ebiohippo.com\/products\/ki-67-antibody-mki67-bha17135866","provider":"BioHippo","version":"1.0","type":"link"}