{"product_id":"kif7-antibody-kinesin-like-protein-kif7-bha17135438","title":"KIF7 Antibody \/ Kinesin-like protein KIF7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eKIF7 Antibody \/ Kinesin-like protein KIF7 is a anti-KIF7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e KIF7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eKIF7 antibody detects Kinesin-like protein KIF7, a microtubule-associated motor protein that plays a crucial role in Hedgehog signaling and ciliary function. KIF7 is a member of the kinesin superfamily and acts as a regulator of microtubule dynamics rather than as a transport motor. The KIF7 antibody is commonly used in developmental biology and cilia research to explore signal transduction, cytoskeletal regulation, and morphogenesis.\u003cbr\u003e\u003cbr\u003eKIF7 is encoded by the KIF7 gene located on human chromosome 15q26.1. The protein is approximately 151 kilodaltons and contains an N-terminal motor domain, a coiled-coil stalk, and a C-terminal tail region required for signaling complex assembly. KIF7 localizes to the primary cilium and functions as a scaffold controlling the balance between active and repressive forms of the transcription factor GLI in response to Hedgehog pathway activation.\u003cbr\u003e\u003cbr\u003eThe KIF7 antibody detects a 134 kilodalton protein by western blot and reveals distinct ciliary tip staining by immunofluorescence. KIF7 regulates microtubule stability and controls ciliary length, essential for proper Hedgehog signal transduction. It restrains GLI2 and GLI3 activity by preventing excessive microtubule polymerization at the ciliary tip and recruiting pathway regulators such as SUFU.\u003cbr\u003e\u003cbr\u003eLoss-of-function mutations in KIF7 cause ciliopathies, including Joubert syndrome and hydrolethalus syndrome, characterized by brain malformations and defective patterning during embryogenesis. Aberrant KIF7 signaling disrupts tissue polarity and organ development, while partial reduction contributes to tumorigenesis by allowing uncontrolled Hedgehog pathway activation.\u003cbr\u003e\u003cbr\u003eKIF7 is an essential modulator of ciliary signaling and structural organization, integrating microtubule mechanics with developmental cues.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=KIF7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=KIF7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=KIF7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/KIF7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=KIF7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291576685,"sku":"FY12535","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_afd59d98-5623-455d-8128-a7936360360a.jpg?v=1782237013","url":"https:\/\/www.ebiohippo.com\/products\/kif7-antibody-kinesin-like-protein-kif7-bha17135438","provider":"BioHippo","version":"1.0","type":"link"}