{"product_id":"kir3dl2-il-2-reporter-lentivirus-bhv19400273","title":"KIR3DL2\/IL-2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eKIR3DL2 is a killer-cell immunoglobulin-like receptor expressed on natural killer cells and subsets of T cells. As an inhibitory KIR, it carries cytoplasmic ITIM motifs that, upon engagement of HLA class I ligands, recruit phosphatases to dampen cytotoxic signaling. This inhibitory function helps NK cells distinguish healthy from abnormal cells and contributes to self-tolerance and the regulation of immune activation. KIR3DL2 is also of clinical interest as a marker and target on certain malignant T cells. Studying KIR3DL2 signaling provides insight into NK cell regulation, immune surveillance, and the development of antibody-based immunotherapies.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe KIR3DL2\/IL-2 Reporter Lentivirus is a two-component immunotherapy reporter system for studying the inhibitory receptor KIR3DL2 in NK cell regulation. One lentivirus constitutively expresses the human KIR3DL2 receptor; the second carries an IL-2-responsive dual reporter combining secreted Gaussia luciferase with a fluorescent protein (GFP or RFP). Ligand engagement of KIR3DL2 suppresses IL-2-driven reporter expression, while blocking antibodies restore the signal, providing a quantitative readout of receptor inhibition. Sequential transduction with antibiotic or fluorescence selection generates a dual-stable reporter cell line. The system is used to evaluate NK cell responses, immune regulation, and KIR3DL2-targeting therapeutics. Supplied as high-titer, VSV-G pseudotyped lentiviral particles optimized for difficult-to-transfect cells, including primary and thawed NK cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human KIR3DL2 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251618013549,"sku":"TRV-0025-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_bd29932a-2a59-4d91-ab1f-518f4c6c8ec1.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/kir3dl2-il-2-reporter-lentivirus-bhv19400273","provider":"BioHippo","version":"1.0","type":"link"}