{"product_id":"klf4-reporter-lentivirus-bhv19400243","title":"KLF4 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eKLF4 (Krüppel-like factor 4) is a zinc-finger transcription factor that binds GC-rich and CACCC DNA elements to activate or repress target genes depending on cellular context. It regulates cell proliferation, differentiation, and tissue homeostasis in epithelial and other tissues, and it is one of the classic reprogramming factors used, with OCT4, SOX2, and MYC, to generate induced pluripotent stem cells. KLF4 contributes to the pluripotency network, in part through regulation of genes such as NANOG. In many epithelial cancers, including colorectal cancer, KLF4 is reported to act as a tumor suppressor, making it an important target in stem cell, developmental, and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe KLF4 Reporter Lentivirus is a transcription-factor reporter system for detecting KLF4-regulated gene expression in mammalian cells. The construct uses tandem repeats of KLF4 DNA-binding elements derived from the human NANOG promoter, coupled to a minimal promoter that drives a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter allow generation of stable polyclonal reporter cell lines with readout by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding the variability of transient transfection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on KLF4 in cellular reprogramming, differentiation, and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the KLF4 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"KLF4-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251620536685,"sku":"LTV-0136-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362086773101,"sku":"LTV-0136-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362086805869,"sku":"LTV-0136-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"KLF4-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362086838637,"sku":"LTV-0136-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362086871405,"sku":"LTV-0136-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362086904173,"sku":"LTV-0136-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362086936941,"sku":"LTV-0136-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"KLF4-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362086969709,"sku":"LTV-0136-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"KLF4-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362087002477,"sku":"LTV-0136-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"KLF4-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362087035245,"sku":"LTV-0136-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"KLF4-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362087068013,"sku":"LTV-0136-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/klf4-2.jpg?v=1776934219","url":"https:\/\/www.ebiohippo.com\/products\/klf4-reporter-lentivirus-bhv19400243","provider":"BioHippo","version":"1.0","type":"link"}