{"product_id":"krt20-antibody-cytokeratin-20-ck20-bha17136273","title":"KRT20 Antibody \/ Cytokeratin 20 \/ CK20","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eKRT20 Antibody \/ Cytokeratin 20 \/ CK20 is a anti-KRT20 Rabbit antibody Recombinant Rabbit Monoclonal clone CDI-11 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e KRT20\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone CDI-11, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eKRT20 antibody detects Cytokeratin 20, an intermediate filament protein encoded by the KRT20 gene on chromosome 17q21.2. Cytokeratin 20 (CK20) is a member of the type I keratin family and is expressed primarily in epithelial cells of the gastrointestinal tract, urothelium, and Merkel cells of the skin. CK20 provides structural stability to epithelial cells and serves as a diagnostic marker in pathology for identifying tissue origin and differentiation status in epithelial tumors. Its expression pattern is especially valuable for distinguishing colorectal, gastric, and bladder carcinomas.\u003cbr\u003e\u003cbr\u003eStructurally, CK20 is a 46 kDa cytoskeletal protein composed of a central alpha-helical rod domain flanked by non-helical head and tail domains, allowing filament assembly with type II keratins such as KRT8. CK20 belongs to the cytokeratin family of intermediate filaments that provide mechanical resilience and maintain epithelial integrity. Co-localization studies show CK20 distributed in the cytoplasm and perinuclear region, forming filamentous networks characteristic of simple epithelia.\u003cbr\u003e\u003cbr\u003eFunctionally, CK20 supports epithelial cell shape, polarity, and resistance to mechanical stress. It interacts with desmosomal and hemidesmosomal components to anchor the cytoskeleton to cell junctions, maintaining tissue architecture. CK20 also participates in cellular differentiation programs, serving as a marker of terminal differentiation in gastrointestinal epithelia and Merkel cells. In the urothelium, CK20 expression increases during tissue repair and regeneration.\u003cbr\u003e\u003cbr\u003eClinically, CK20 serves as an important immunohistochemical marker in cancer diagnostics. It is strongly expressed in colorectal adenocarcinomas, transitional cell carcinomas of the bladder, and some gastric cancers, while absent in most lung, breast, and prostate carcinomas. Combined with CK7 expression patterns, CK20 staining aids in identifying metastatic tumor origin. Dysregulation of KRT20 gene expression has been linked to epithelial barrier dysfunction and tumor progression. Pathway involvement includes cytoskeletal organization, epithelial differentiation, and stress response signaling.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using KRT20 antibody reveals strong cytoplasmic filamentous localization in gastrointestinal and urothelial epithelia. The KRT20 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=KRT20 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=KRT20 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=KRT20 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/KRT20 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=KRT20+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047323951469,"sku":"FY13371","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7c1432ea-c186-496f-8b54-e6a2a6ffdc57.jpg?v=1772019438","url":"https:\/\/www.ebiohippo.com\/products\/krt20-antibody-cytokeratin-20-ck20-bha17136273","provider":"BioHippo","version":"1.0","type":"link"}