{"product_id":"ktyi-stable-expressing-mouse-keratinocyte-cell-line-bhc10901281","title":"KtyI Stable Expressing Mouse Keratinocyte Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eKtyI Stable Expressing Mouse Keratinocyte Cell Line are spontaneously immortalized cells. The cells express keratins (ktyl) and may be used in conjunction with Ktly Stable Knockout Mouse Keratinocyte Cell Line (T6293) to study the affects of the presence and absence of keratins.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e KtyI Stable Expressing Mouse Keratinocyte Cell Line is supplied as an engineered cell line derived from Mouse skin.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. DMEM\/Ham’s F12, low Calcium (50µM) (Biochrom) + 10% chelexed fetal calf serum (PAA, A15-151) + 2 mM Glutamax (Invitrogen, 35050-038) + 1X Pyruvate (PAA, S11-003) + 0.18mM Adenin (Sigma, A8626-5G) + 10 ng\/ml EGF (Invitrogen 53003-018) + 2.5 µg\/ml insulin (Sigma-Aldrich, I-9278) + 0.5 µl Hydroxycortison (Sigma-Aldrich, H4001-1G) + 10-⁻¹⁰ M Cholera toxin (Sigma,C-8052) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Selection with 0.1 µg\/ml Puromycin (G264)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in skin biology, phenotype comparison, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in skin biology, phenotype comparison, and assay development. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. DMEM\/Ham’s F12, low Calcium (50µM) (Biochrom) + 10% chelexed fetal calf serum (PAA, A15-151) + 2 mM Glutamax (Invitrogen, 35050-038) + 1X Pyruvate (PAA, S11-003) + 0.18mM Adenin (Sigma, A8626-5G) + 10 ng\/ml EGF (Invitrogen 53003-018) + 2.5 µg\/ml insulin (Sigma-Aldrich, I-9278) + 0.5 µl Hydroxycortison (Sigma-Aldrich, H4001-1G) + 10-⁻¹⁰ M Cholera toxin (Sigma,C-8052) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Selection with 0.1 µg\/ml Puromycin (G264)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 30,000\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180522365293,"sku":"T6294","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/T6294_Morphology.png?v=1774957814","url":"https:\/\/www.ebiohippo.com\/products\/ktyi-stable-expressing-mouse-keratinocyte-cell-line-bhc10901281","provider":"BioHippo","version":"1.0","type":"link"}