{"product_id":"lag-3-antibody-lymphocyte-activation-gene-3-protein-cd223-bha17135600","title":"LAG-3 Antibody \/ Lymphocyte activation gene 3 protein \/ CD223","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLAG-3 Antibody \/ Lymphocyte activation gene 3 protein \/ CD223 is a anti-LAG-3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31L58 supplied in Liquid format. Recommended for workflows such as Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LAG-3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31L58, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IF, IHC, ICC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLAG-3 antibody detects lymphocyte activation gene 3 protein, an immune checkpoint receptor encoded by the LAG3 gene. LAG-3 is expressed on activated T cells, natural killer cells, and plasmacytoid dendritic cells. It binds MHC class II molecules and negatively regulates T cell proliferation and activation. This checkpoint function maintains immune tolerance and prevents autoimmunity, but also contributes to immune evasion in cancer.\u003cbr\u003e\u003cbr\u003eLAG-3 antibody is widely applied in immunology, oncology, and translational research. In cancer, LAG-3 expression marks exhausted T cells within the tumor microenvironment. In chronic infection, sustained LAG-3 expression dampens antiviral responses. By detecting LAG-3, researchers can evaluate immune exhaustion, tolerance mechanisms, and therapeutic checkpoint blockade strategies.\u003cbr\u003e\u003cbr\u003e Flow cytometry with LAG-3 antibody identifies exhausted or regulatory T cell subsets. Immunohistochemistry maps LAG-3 expression in tumors and inflamed tissues, while immunofluorescence highlights surface distribution on lymphocytes. Western blotting confirms expression in activated immune cell lysates. These methods enable detailed characterization of immune checkpoint biology.\u003cbr\u003e\u003cbr\u003eLAG-3 has therapeutic relevance as a target for immune checkpoint inhibitors. Antibodies blocking LAG-3 restore T cell function and enhance antitumor immunity. Clinical trials are evaluating LAG-3 blockade alone and in combination with PD-1 inhibitors. By applying LAG-3 antibody, scientists can monitor expression as a biomarker for patient selection and therapeutic response.\u003cbr\u003e\u003cbr\u003eLAG-3 is also involved in autoimmune disease and transplantation. It modulates T regulatory cell function and dendritic cell activation, influencing tolerance and graft survival. Studying LAG-3 with antibody based methods provides insights into balancing immunity and tolerance in clinical settings.\u003cbr\u003e\u003cbr\u003eLAG-3 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LAG-3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LAG-3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LAG-3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LAG-3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LAG-3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047297147245,"sku":"FY12697","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3d1571bf-22d7-4c71-83da-f4bfe2af533b.jpg?v=1772019330","url":"https:\/\/www.ebiohippo.com\/products\/lag-3-antibody-lymphocyte-activation-gene-3-protein-cd223-bha17135600","provider":"BioHippo","version":"1.0","type":"link"}