{"product_id":"lamin-b2-antibody-lmnb2-bha17135240","title":"Lamin B2 Antibody \/ LMNB2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLamin B2 Antibody \/ LMNB2 is a anti-B2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Rat. Reported localization: Nucleus lamina.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e B2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe Lamin B2 antibody targets lamin B2, an essential component of the nuclear lamina. Lamins are type V intermediate filament proteins that provide structural support to the nuclear envelope and participate in chromatin organization, DNA replication, transcription, and nuclear stability. Lamin B2, encoded by the LMNB2 gene, localizes to the inner nuclear membrane where it forms a filamentous meshwork underlying the nuclear envelope. This protein is crucial for maintaining nuclear shape, ensuring proper chromosome segregation during mitosis, and anchoring chromatin domains. The Lamin B2 antibody allows researchers to investigate the abundance, localization, and regulatory functions of this protein in diverse biological contexts.\u003cbr\u003e\u003cbr\u003eLamin B2 is expressed in most somatic tissues and plays particularly important roles in proliferating cells. Mutations in LMNB2 have been associated with neurological disorders such as progressive myoclonus epilepsy, underscoring the importance of Lamin B2 for brain development and function. Studies have also implicated changes in lamin expression in processes such as aging, cancer, and viral infection, making antibodies against these proteins highly valuable for mechanistic investigations. The Lamin B2 antibody provides a reliable means of probing nuclear envelope biology and identifying changes that accompany disease progression.\u003cbr\u003e\u003cbr\u003eIn experimental applications, the Lamin B2 antibody has broad utility. Western blotting can reveal protein levels and detect shifts in size due to post-translational modifications. Immunofluorescence microscopy allows visualization of nuclear lamina organization and potential defects in nuclear envelope integrity. Immunohistochemistry enables tissue-level assessment of lamin expression, which may vary between normal and diseased tissues. Given lamin involvement in DNA replication and repair, the Lamin B2 antibody is also relevant in studies of genomic stability and cellular responses to stress. These uses highlight the versatility of the reagent for both basic and translational research.\u003cbr\u003e\u003cbr\u003eThe structural role of lamin proteins makes them targets of interest in cancer research. Altered lamin expression has been linked to changes in nuclear morphology, a hallmark of many tumor cells. Lamin B2 dysregulation could influence tumor progression by affecting nuclear stiffness, gene expression, and mechanotransduction.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=B2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=B2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=B2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/B2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=B2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285121389,"sku":"FY12337","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d2b6c5f9-e8fb-4924-a837-808ecb7ae838.jpg?v=1782236995","url":"https:\/\/www.ebiohippo.com\/products\/lamin-b2-antibody-lmnb2-bha17135240","provider":"BioHippo","version":"1.0","type":"link"}