{"product_id":"lat-antibody-linker-for-activation-of-t-cells-bha17135894","title":"Lat Antibody \/ Linker for activation of T cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLat Antibody \/ Linker for activation of T cells is a anti-Lat Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat. Reported localization: Plasma membrane, Golgi.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Lat\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLAT antibody detects Linker for activation of T cells, a critical transmembrane adaptor protein that functions as a central scaffold in T-cell receptor (TCR) signaling. The UniProt recommended name is Linker for activation of T cells (LAT). This protein coordinates the assembly of multiprotein complexes that mediate T-cell activation, proliferation, and immune response regulation. LAT is indispensable for coupling TCR engagement to downstream signaling cascades that drive cytokine production and effector cell differentiation.\u003cbr\u003e\u003cbr\u003eFunctionally, LAT antibody identifies a 233-amino-acid membrane protein that localizes to lipid rafts of the plasma membrane. Upon TCR stimulation, the Src-family kinase Lck and the ZAP-70 tyrosine kinase phosphorylate multiple tyrosine residues on LAT, creating docking sites for SH2-domain-containing proteins such as Grb2, PLC-gamma1, and Gads. These interactions recruit downstream effectors that activate calcium flux, MAPK signaling, and transcriptional pathways including NFAT, NF-kappaB, and AP-1. Through these mechanisms, LAT orchestrates the signal amplification necessary for full T-cell activation.\u003cbr\u003e\u003cbr\u003eThe LAT gene is located on chromosome 16p11.2 and encodes a single-pass type III transmembrane protein with a short extracellular domain and a cytoplasmic tail rich in tyrosine residues. The LAT scaffold acts as an organizational hub, integrating signals from the TCR, co-stimulatory receptors, and cytokine receptors. LAT phosphorylation dynamics are tightly regulated to prevent aberrant T-cell activation and autoimmunity. In developing thymocytes, LAT signaling contributes to both positive and negative selection, shaping the mature T-cell repertoire.\u003cbr\u003e\u003cbr\u003eDefects in LAT expression or phosphorylation impair T-cell development and lead to severe combined immunodeficiency-like phenotypes. Conversely, gain-of-function mutations or dysregulated LAT signaling can cause hyperactivation syndromes, lymphoproliferative disorders, or autoimmunity. LAT also functions in mast cells, natural killer cells, and platelets, where it mediates Fc receptor-dependent signaling and degranulation responses.\u003cbr\u003e\u003cbr\u003eLAT antibody is widely used in immunology, signal transduction, and cell biology research. It is suitable for western blotting, flow cytometry, and immunofluorescence to examine TCR-induced phosphorylation and signal complex formation. Researchers employ this antibody to dissect T-cell activation pathways and study immune regulation at the molecular level. LAT serves as a key marker of functional TCR signaling in both primary immune cells and model systems.\u003cbr\u003e\u003cbr\u003eStructurally, LAT contains a cytoplasmic region with multiple phosphorylation sites that function as SH2-binding motifs for signaling partners. It forms large signalosomes through multivalent interactions that promote phase-separated clusters at the membrane.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Lat - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Lat - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Lat - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Lat - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Lat+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308714349,"sku":"FY12992","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_70dfd706-f24e-4ae8-847c-d62f4c273aee.jpg?v=1782237075","url":"https:\/\/www.ebiohippo.com\/products\/lat-antibody-linker-for-activation-of-t-cells-bha17135894","provider":"BioHippo","version":"1.0","type":"link"}