{"product_id":"lgals3-antibody-galectin-3-gal3-bha17135372","title":"LGALS3 Antibody \/ Galectin 3 \/ GAL3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLGALS3 Antibody \/ Galectin 3 \/ GAL3 is a anti-LGALS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LGALS3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLGALS3 antibody detects Galectin-3, a beta-galactoside-binding lectin involved in cell adhesion, immune modulation, apoptosis regulation, fibrosis, and tumor progression. Galectin-3 functions as both an intracellular regulator and an extracellular signaling molecule, mediating diverse processes such as inflammation, angiogenesis, and cell-matrix interaction. The LGALS3 antibody is extensively used in studies of cancer, cardiovascular disease, and immune disorders where Galectin-3 serves as a biomarker and functional effector in pathological remodeling.\u003cbr\u003e\u003cbr\u003eGalectin-3 is encoded by the LGALS3 gene located on human chromosome 14q22.3. The protein contains a carbohydrate recognition domain that binds beta-galactoside residues on glycoproteins and an N-terminal domain enabling oligomerization. This structure allows Galectin-3 to form lattices at the cell surface, influencing receptor clustering and signaling. It is expressed in epithelial cells, macrophages, fibroblasts, and endothelial cells, and can be secreted via a non-classical pathway into the extracellular space. Once outside the cell, it modulates adhesion and migration, contributing to wound healing and fibrosis.\u003cbr\u003e\u003cbr\u003eThe LGALS3 antibody is particularly useful for detecting Galectin-3 expression in tumor and fibrotic tissues. Western blot typically shows an ~26 kilodalton band, while immunostaining reveals cytoplasmic, nuclear, or membrane-associated localization depending on cellular context. Overexpression of Galectin-3 is associated with aggressive tumor phenotypes, including resistance to apoptosis and enhanced metastasis. Mechanistically, Galectin-3 interacts with cell-surface receptors such as integrins and growth factor receptors, amplifying signaling through the phosphoinositide 3-kinase\/AKT and transforming growth factor beta (TGF-beta) pathways. This dual role in intracellular survival signaling and extracellular communication underscores its complexity as a therapeutic target.\u003cbr\u003e\u003cbr\u003eBeyond oncology, Galectin-3 contributes to fibrotic progression in the heart, kidneys, and liver. It promotes fibroblast activation, collagen deposition, and tissue stiffening, leading to organ dysfunction. Elevated plasma Galectin-3 levels are now used clinically as a prognostic biomarker for heart failure. In immune regulation, Galectin-3 modulates macrophage polarization, promotes neutrophil adhesion, and suppresses T-cell receptor signaling, linking innate and adaptive immune responses.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LGALS3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LGALS3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LGALS3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LGALS3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LGALS3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289708909,"sku":"FY12469","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e258f688-7e25-4ca6-b3e2-9e698cf41e94.jpg?v=1782237012","url":"https:\/\/www.ebiohippo.com\/products\/lgals3-antibody-galectin-3-gal3-bha17135372","provider":"BioHippo","version":"1.0","type":"link"}