{"product_id":"lig4-antibody-dna-ligase-4-bha17136031","title":"LIG4 Antibody \/ DNA Ligase 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLIG4 Antibody \/ DNA Ligase 4 is a anti-LIG4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LIG4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLIG4 antibody detects DNA ligase 4, an essential enzyme that catalyzes the final ligation step in non-homologous end joining (NHEJ) DNA repair. The UniProt recommended name is DNA ligase 4 (LIG4). This enzyme joins DNA double-strand breaks generated during recombination, replication stress, or DNA damage, preserving genome stability.\u003cbr\u003e\u003cbr\u003eFunctionally, LIG4 antibody identifies a 911-amino-acid ATP-dependent DNA ligase that seals nicks in double-stranded DNA by catalyzing phosphodiester bond formation. LIG4 operates in complex with XRCC4 and XLF\/Cernunnos to align DNA ends and complete repair during NHEJ. It also plays a critical role in V(D)J recombination in lymphocytes, supporting immune diversity.\u003cbr\u003e\u003cbr\u003eThe LIG4 gene is located on chromosome 13q33.3 and is ubiquitously expressed in proliferating tissues. Its expression is highest in immune cells, where DNA recombination occurs frequently. Proper function of LIG4 ensures efficient DNA repair and prevents chromosomal translocations and mutagenesis.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in LIG4 cause Ligase IV syndrome, characterized by microcephaly, developmental delay, immunodeficiency, and radiosensitivity. Deficiency in LIG4 impairs DNA double-strand break repair, leading to genomic instability and increased cancer susceptibility. Research using LIG4 antibody aids in studies of DNA repair, recombination, and genomic maintenance.\u003cbr\u003e\u003cbr\u003eLIG4 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect ligase enzymes in DNA damage response pathways. \u003cbr\u003e\u003cbr\u003eStructurally, DNA ligase 4 contains an N-terminal DNA-binding domain, an adenylation domain that binds AMP, and a C-terminal XRCC4-interacting region necessary for NHEJ complex formation. This antibody enables detailed analysis of LIG4's enzymatic activity in genome integrity and repair pathways.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LIG4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LIG4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LIG4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LIG4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LIG4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312449901,"sku":"FY13129","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_18da3798-77ac-4c24-9b82-ea7e0e799f39.jpg?v=1782237088","url":"https:\/\/www.ebiohippo.com\/products\/lig4-antibody-dna-ligase-4-bha17136031","provider":"BioHippo","version":"1.0","type":"link"}