{"product_id":"limitless-elz-fusion-kit-bht15200030","title":"Limitless™ ELZ Fusion Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eCloning Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eLimitless™ ELZ Fusion is a seamless, homology-based directional cloning kit that assembles fragments carrying 15–40 bp of vector homology in a single 37°C reaction, and that allows insertion of target genes at any position in any vector. The Limitless™ ELZ Fusion Seamless Cloning Kit is extremely simple to operate, requiring only linearization of the vector, mixing with PCR-amplified fragments that are completely homologous to the vector (15–40 bp) and reacting at 37°C, followed by direct transformation. The manufacturer states a positive clone rate of over 95%.\u003c\/p\u003e\n\u003cp\u003eThe kit supplies a 5× Limitless™ ELZ Fusion Mix for 25 reactions. A 20 µL reaction combines the linearized vector and the insert at an insert-to-vector molar ratio of 2:1 to 5:1 with 4 µL of 5× Mix and water, is incubated at 37°C for 30 minutes and then chilled on ice for 5 minutes. 10 µL of the reaction is used for transformation and the remaining 10 µL can be stored at 4°C or -20°C.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSingle 5× reaction mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCloning method\u003c\/th\u003e\n\u003ctd\u003eSeamless, homology-based directional cloning\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHomology arm length\u003c\/th\u003e\n\u003ctd\u003e15–40 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eInsert-to-vector molar ratio\u003c\/th\u003e\n\u003ctd\u003e2:1 to 5:1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction volume\u003c\/th\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction time\u003c\/th\u003e\n\u003ctd\u003e30 minutes at 37°C, then 5 minutes on ice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePositive clone rate\u003c\/th\u003e\n\u003ctd\u003eOver 95%, as stated by the manufacturer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e25 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5× Limitless™ ELZ Fusion Mix\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSeamless cloning of PCR-amplified fragments into linearized vectors\u003c\/li\u003e\n\u003cli\u003eInsertion of target genes at any position in a vector\u003c\/li\u003e\n\u003cli\u003eInsertion of multiple target genes into one vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C. The shelf life is 1 year. Shipping: Blue ice.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDesign primers so that the amplified insert carries 15–40 bp of sequence completely homologous to the vector ends, and purify the insert before assembly.\u003c\/li\u003e\n\u003cli\u003eLinearize the vector by restriction digestion or PCR before setting up the reaction.\u003c\/li\u003e\n\u003cli\u003eChill the reaction on ice for 5 minutes after the 37°C incubation.\u003c\/li\u003e\n\u003cli\u003eStore the unused 10 µL of the reaction at 4°C or -20°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eLEF01\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200030\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"25 rxn","offer_id":53965512638829,"sku":"LEF01-25RXN","price":156.0,"currency_code":"USD","in_stock":true},{"title":"50 rxn","offer_id":53965515063661,"sku":"LEF01-50RXN","price":234.0,"currency_code":"USD","in_stock":true},{"title":"25 rxn x 5","offer_id":53965515096429,"sku":"LEF01-25RXNX5","price":0.0,"currency_code":"USD","in_stock":true},{"title":"50 rxn x 5","offer_id":53965515129197,"sku":"LEF01-50RXNX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LEF01-a-1.jpg?v=1789419121","url":"https:\/\/www.ebiohippo.com\/products\/limitless-elz-fusion-kit-bht15200030","provider":"BioHippo","version":"1.0","type":"link"}