{"product_id":"lipod293-in-vitro-dna-transfection-reagent-bht2200061","title":"LipoD293™ In Vitro DNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eSignaGen's innovative, proprietary lipid conjugation technology underlies LipoD293™ (Ver. II), whose design and proprietary enhancers aim at highly efficient DNA transfection in HEK293 cells along with other mammalian cell types. From this second-generation liposome-based DNA transfection reagent SignaGen reports exceptionally high transfection efficiency in HEK293-derived cells and in many other mammalian cells, cytotoxicity staying low throughout.\u003c\/p\u003e\n\u003cp\u003eRefined chemistry separates it from the previous formulation and brings a 3–4 fold improvement in gene delivery efficiency. Approximately 666 transfections in 24-well plates, or 333 in 6-well plates, can be run from one 1.0 mL vial of LipoD293™ (Ver. II) Reagent.\u003c\/p\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD-Enhanced.jpg?v=1790173499\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – schematic of enhanced gene delivery\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 1. Schematic illustration of enhanced gene delivery mediated by LipoD293™ (Ver. II).\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under recommended conditions, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePreferred option for cells that are hard to transfect\u003c\/li\u003e\n\u003cli\u003eSupports exceptionally high viral titers\u003c\/li\u003e\n\u003cli\u003eHighly effective with very large DNA constructs (up to 180 kb)\u003c\/li\u003e\n\u003cli\u003eWorks equally well in suspension HEK293 cells (e.g., 293F, 293H)\u003c\/li\u003e\n\u003cli\u003eEnables high-level recombinant protein expression\u003c\/li\u003e\n\u003cli\u003eHigher transfection efficiency when serum and antibiotics are present in 293 cells\u003c\/li\u003e\n\u003cli\u003eExceptional results with both single-DNA transfection and multi-DNA co-transfection\u003c\/li\u003e\n\u003cli\u003eHighly cost-effective transfection option\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD293Comparison2.jpg?v=1790173499\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – HepG2 GFP transfection vs L2K and Fugene HD; serum\/antibiotic effect\" loading=\"lazy\"\u003e\u003cfigcaption\u003eComparison of transfection efficiency of LipoD293™ reagent (Ver. II) vs. lipofectamine 2000 (L2K) and Fugene HD on HepG2 cells. Right Panel: Comparison of transfection efficiency of LipoD293 (Ver. II) with Lipofectamine 2000 (L2K), and Fugene HD on HepG2 cells. GFP DNA (pEGFP-N3) was transfected with different transfection reagents per the manufacturer’s protocols to HepG2 cell (cultured on collagen-pretreated dishes). GFP-positive cell (%) and fluorescence intensity were detected by passing through FACS 48 hours post-transfection Left Panel: presence of serum and antibiotics enhances LipoD293 (Ver. II) efficiency on HepG2 cells. HepG2 cell (grown on collagen-treated dishes) was transfected with three different conditions——-serum and antibiotics free, presence of 10% serum and antibiotics, followed by removal 5 hours post transfection, and presence of 10% serum and antibiotics without removal 5 hours post transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD293Comparison.jpg?v=1790173498\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – CHO transfection vs L2K, TransIT and Fugene 6, with price comparison\" loading=\"lazy\"\u003e\u003cfigcaption\u003eComparison of transfection efficiency of LipoD293™ reagent (Ver. II) vs. lipofectamine 2000 (L2K), TransIT and Fugene 6 on CHO cells. Right Panel: Comparison of transfection efficiency of LipoD293 (Ver. II) with Lipofecatmine 2000 (L2K), TransIT and Fugene 6 on CHO cells. DNAs encoding Renilla luciferase (phRL-CMV) and GFP (pEGFP-N3) were transfected with different DNA transfection reagent per the manufacturer’s protocols. Renilla luciferase activity and GFP fluorescence were detected with Renilla Assay System and a Nikon Eclipse fluorescent microscope, respectively, 24 hours post-transfection. Left Panel: Comparison of price ($\/1.0 ml vial) of LipoD293 versus those of Lipofecatmine 2000 (L2K), TransIT, and Fugene 6. All the prices were collected from the manufacturers’ websites.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD293-RASMC.jpg?v=1790173499\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – GFP in primary rat aortic smooth muscle cells vs Lipofectamine 2000\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of the transfection efficiency of LipoD293™ reagent with Lipofectamine 2000 (L2K) on a hard-to-transfect cell, primary rat aortic smooth muscle cells. The rat aortic smooth muscle cells were prepared and transfected with pEGFP-N3 by LipoD293™ reagent (left panel) and Lipofectamine 2000 (L2K, right panel), respectively per the manufacturers’ protocols. The transfection efficiency was evaluated by detecting GFP fluorescence with a Nikon Eclipse 2000 microscope 24 hours post-transfection. The above pictures were kindly provided by Dr. Nickolai Dulin of the Section of Pulmonary and Critical Care, University of Chicago.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LNcapComparison.jpg?v=1790173499\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – GFP co-transfection in LNCap cells vs Fugene HD\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of the transfection efficiency of LipoD293™ reagent with Fugene HD on hard-to-transfect cells, LNCap cells. The LNCap cells were grown according to ATCC recommended procedures and co-transfected with pBabe-hygro-SSeCKs (1.5 ug) and pEGFP-N3 (0.5 ug) per well (6-well plate), LipoD293™ reagent (left panel) and Fugene HD (right panel), respectively per the manufacturers’ protocols. The transfection efficiency was evaluated by detecting GFP fluorescence with a Nikon Eclipse 2000 microscope 24 hours post-transfection. The above pictures were kindly provided by Dr. Lyn Gao of Roswell Park Cancer Institute.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD293-HS.jpg?v=1790173499\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – GFP in HepG2 and β-galactosidase in SaoS-2 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eTwo examples showing the exceptional efficiency of LipoD293™ reagent on hard-to-transfect cells like HepG2 and SaoS-2 cells. HepG2 and SaoS-2 cells in 95% confluency were transfected with pEGFP-N3 and pSV-β-galactosidase DNAs, respectively in the presence of serum\/antibiotics. The efficiency was checked 48 hours post-transfection by Zeiss 510 Confocal Microscopy and β-galactosidase staining kit, respectively.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD293.jpg?v=1790173498\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – GFP transfection in HEK293 cells, DIC and FITC imaging\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-293fecin.jpg?v=1790173498\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – HEK293 cells transfected with 293fectin™ for comparison, DIC and FITC imaging\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of the transfection efficiency of LipoD293™ reagent with 293fectin on HEK293 cells. HEK293 cells transfected with the pEGFP-C1 plasmid using LipoD293™ In Vitro DNA Transfection Reagent (Ver. II) (upper panel) and the most popular brand product 293fectin™ of Invitrogen (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD293_vs_293Fectin_III.jpg?v=1790173498\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – GFP-His protein production in 293F cells vs 293fectin, Xfect and Fugene 6\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of LipoD293™ reagent vs. 293fectin, Xfect, and Fugene 6 transfection reagents on protein production with suspension 293F cells. 30 mL of 293F cell cultured in standard culture medium was transfected with pEGFP-6xHis plasmid using LipoD293™ In Vitro DNA Transfection Reagent (Ver. II) (20 ug plasmid DNA), 293Fectin (30 ug plasmid DNA), Xfect (30 ug plasmid DNA) and Fugene 6 (30 ug plasmid DNA) per manufacturers’ standard transfection protocols. GFP fluorescence was visualized 48 hours post-transfection (left panel) with A for LipoD293, B for 293fectin, C for Xfect, and D for Fugene 6. The 6xHis tagged GFP protein was then purified via Ni-NTA affinity column. 5 μL of 1st elution fraction was resolved on SDS-PAGE, followed by Coomassie Brilliant Blue staining (right upper panel E) with lane 1 for LipoD293, lane 2 for protein marker, lane 3 for Xfect, lane 4 for 293fectin, and lane 5 for Fugene 6. The protein yield was quantified via a spectrometer (right lower panel F).\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD-LV-13.jpg?v=1790173499\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – lentivirus production in 293FT cells vs Lipofectamine LTX, titer by FACS\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD-LV-14.jpg?v=1790173499\" alt=\"LipoD293™ In Vitro DNA Transfection Reagent – lentivirus production in 293FT cells vs Lipofectamine LTX, titer by FACS\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of LipoD293™ (Ver. II) and Lipofecatmine LTX (LTX) on the generation of Lentivirus (LV). Three cDNAs were co-transfected with LipoD293™ (Ver. II) and Lipofectamine LTX (LTX) into 293FT cells. A GFP vector, pHR-SIN-cppt-CMVEWP, was used to determine the titer of LV. 1×105 293F cells per well were plated into a 24-well plate followed by the addition of different amounts of the vector supernatant, 1 microliter (upper panel) and 10 microliters (lower panel), respectively. 5 days later, the cells were passed with FACS. The numbers in the upper right corner indicate the percentage of transduced cells. The titers of LV generated with LipoD293™ and L2K were quantified to be 8×10^6 and 3×10^6 tu\/ml, respectively\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900758893,"sku":"SL100668-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042906722669,"sku":"SL100668-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042906755437,"sku":"SL100668-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042906788205,"sku":"SL100668-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoD293_png_153f3055-9468-435e-aa44-d0e8de0c3016.webp?v=1790175949","url":"https:\/\/www.ebiohippo.com\/products\/lipod293-in-vitro-dna-transfection-reagent-bht2200061","provider":"BioHippo","version":"1.0","type":"link"}