{"product_id":"lmod3-antibody-leiomodin-3-bha17135873","title":"LMOD3 Antibody \/ Leiomodin 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLMOD3 Antibody \/ Leiomodin 3 is a anti-LMOD3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LMOD3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLMOD3 antibody detects Leiomodin-3, a muscle-specific actin-binding protein that regulates thin filament assembly and sarcomere organization. The UniProt recommended name is Leiomodin-3 (LMOD3), a member of the leiomodin and tropomodulin family of actin filament regulators. LMOD3 is predominantly expressed in skeletal and cardiac muscle, where it acts as a potent nucleator and stabilizer of actin filaments critical for muscle contraction and structural integrity.\u003cbr\u003e\u003cbr\u003eFunctionally, LMOD3 antibody identifies a 579-amino-acid cytoskeletal protein that nucleates the formation of actin filaments by interacting with actin monomers and tropomyosin. Unlike tropomodulins, which cap the pointed ends of actin filaments to prevent elongation, LMOD3 promotes filament growth and organization, ensuring proper sarcomere length and alignment. Its C-terminal leucine-rich and WH2 domains bind actin directly, while an N-terminal region interacts with tropomyosin and other sarcomeric proteins. Through these interactions, LMOD3 maintains filament stability and alignment within the myofibrillar lattice.\u003cbr\u003e\u003cbr\u003eThe LMOD3 gene is located on chromosome 3p14.1 and encodes a protein essential for skeletal muscle development and maintenance. Mutations in LMOD3 cause nemaline myopathy type 10, a congenital disorder characterized by muscle weakness and the presence of nemaline bodies within myofibers. Defective LMOD3 leads to impaired thin filament elongation and disorganized sarcomeres, resulting in reduced muscle contractility and atrophy.\u003cbr\u003e\u003cbr\u003eIn normal muscle physiology, LMOD3 functions alongside leiomodin-1 and leiomodin-2, each serving tissue-specific roles in actin organization. LMOD3 localizes to the pointed ends of thin filaments near the M-line, complementing tropomodulin's capping activity at the same site. The balance between capping and elongation driven by LMOD3 and tropomodulin ensures optimal sarcomere structure and mechanical stability. In cardiac muscle, LMOD3 expression is induced during development and hypertrophic remodeling, indicating roles in both growth and adaptation.\u003cbr\u003e\u003cbr\u003eLMOD3 antibody is commonly used to study muscle biology, sarcomere assembly, and actin cytoskeleton regulation. It is suitable for western blotting, immunofluorescence, and immunohistochemistry to detect LMOD3 expression in skeletal and cardiac tissues. Researchers utilize this antibody to investigate myopathies, cytoskeletal disorders, and mechanisms of muscle fiber regeneration. In vitro studies have shown that overexpression of LMOD3 enhances actin nucleation, while knockdown disrupts filament alignment and leads to myofibrillar disarray.\u003cbr\u003e\u003cbr\u003eStructurally, LMOD3 contains a characteristic leiomodin family domain architecture with N-terminal tropomyosin-binding regions, central coiled-coil motifs, and C-terminal WH2 domains for actin nucleation. It also undergoes phosphorylation-dependent regulation that modulates its actin-binding affinity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LMOD3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LMOD3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LMOD3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LMOD3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LMOD3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308190061,"sku":"FY12971","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8b47c43b-ccb6-4b31-bfd4-81403cf0bb28.jpg?v=1782237069","url":"https:\/\/www.ebiohippo.com\/products\/lmod3-antibody-leiomodin-3-bha17135873","provider":"BioHippo","version":"1.0","type":"link"}