{"product_id":"lrguk-antibody-leucine-rich-repeat-and-guanylate-kinase-domain-containing-protein-bha17135565","title":"LRGUK Antibody \/ Leucine-rich repeat and guanylate kinase domain-containing protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLRGUK Antibody \/ Leucine-rich repeat and guanylate kinase domain-containing protein is a anti-LRGUK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LRGUK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLRGUK antibody detects Leucine-rich repeat and guanylate kinase domain-containing protein, a testis-specific protein that plays a critical role in spermatogenesis and sperm flagellar assembly. LRGUK acts as a scaffolding protein that connects the axoneme and peri-basal body structures necessary for sperm motility and morphology. The LRGUK antibody is widely used in reproductive biology and developmental studies to examine flagellar biogenesis, male fertility, and cytoskeletal organization.\u003cbr\u003e\u003cbr\u003eLRGUK is encoded by the LRGUK gene located on human chromosome 7q22.1. The protein is approximately 1,453 amino acids in length and contains multiple leucine-rich repeats and a C-terminal guanylate kinase-like domain that mediates protein-protein interactions. LRGUK localizes to the distal centriole, connecting piece, and midpiece of developing spermatozoa, where it interacts with structural and motor proteins involved in axonemal extension.\u003cbr\u003e\u003cbr\u003eThe LRGUK antibody detects a 165 kilodalton protein by western blot and shows strong localization in elongating spermatids under immunohistochemistry. LRGUK functions as a molecular linker between microtubules and accessory structures such as the mitochondrial sheath, ensuring proper flagellar alignment and motility. Mutations or knockouts of LRGUK in animal models result in immotile sperm with abnormal tail formation, leading to infertility.\u003cbr\u003e\u003cbr\u003eBeyond the testis, low levels of LRGUK expression have been reported in ciliated epithelial tissues, suggesting additional roles in ciliogenesis. By anchoring structural elements within motile cilia, LRGUK contributes to coordinated movement and fluid transport in the respiratory and reproductive tracts.\u003cbr\u003e\u003cbr\u003eBecause LRGUK serves as a key structural determinant of flagellar integrity and motility, it represents an important marker for studying male reproductive health and cytoskeletal organization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LRGUK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LRGUK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LRGUK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LRGUK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LRGUK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047295312237,"sku":"FY12662","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_72876e3f-90fc-42d5-bc85-7425c5549263.jpg?v=1782237037","url":"https:\/\/www.ebiohippo.com\/products\/lrguk-antibody-leucine-rich-repeat-and-guanylate-kinase-domain-containing-protein-bha17135565","provider":"BioHippo","version":"1.0","type":"link"}