{"product_id":"lrh1-reporter-lentivirus-bhv19400232","title":"LRH1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eLRH1, encoded by NR5A2, is a ligand-regulated nuclear receptor that binds DNA as a monomer. It is co-expressed with the scavenger receptor SR-BI in the liver and ovary, where it plays critical roles in cholesterol metabolism, bile acid synthesis, and lipid transport. LRH1 also regulates metabolism, development, and immune function by sensing changes in blood glucose, lipid abundance, and hormone balance, and it influences cell proliferation in intestinal and hepatic tissues. Because of its central role in metabolic homeostasis and its dysregulation in metabolic disease and cancer, LRH1 is an actively studied target in endocrinology and metabolic research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe LRH1 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of liver receptor homolog 1 (LRH1) transcriptional activity in mammalian cells. The construct uses tandem repeats of consensus LRH1 DNA-binding elements within the SR-BI promoter context to drive reporter expression, so signal reflects endogenous LRH1 activity. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study LRH1 function in cholesterol and bile acid metabolism, lipid homeostasis, and nuclear receptor pharmacology. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the LRH1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"LRH1-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251619324269,"sku":"LTV-0125-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362091229549,"sku":"LTV-0125-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362091262317,"sku":"LTV-0125-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"LRH1-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362091295085,"sku":"LTV-0125-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362091327853,"sku":"LTV-0125-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362091360621,"sku":"LTV-0125-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362091393389,"sku":"LTV-0125-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"LRH1-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362091426157,"sku":"LTV-0125-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"LRH1-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362091458925,"sku":"LTV-0125-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"LRH1-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362091491693,"sku":"LTV-0125-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"LRH1-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362091524461,"sku":"LTV-0125-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LRH1-Reporter-Figure.jpg?v=1776934148","url":"https:\/\/www.ebiohippo.com\/products\/lrh1-reporter-lentivirus-bhv19400232","provider":"BioHippo","version":"1.0","type":"link"}