{"product_id":"lrif1-antibody-ligand-dependent-nuclear-receptor-interacting-factor-1-rif-1-hbix1-c1orf103-bha17136060","title":"LRIF1 Antibody \/ Ligand-dependent nuclear receptor-interacting factor 1 \/ RIF-1 \/ HBiX1 \/ C1orf103","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLRIF1 Antibody \/ Ligand-dependent nuclear receptor-interacting factor 1 \/ RIF-1 \/ HBiX1 \/ C1orf103 is a anti-LRIF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LRIF1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLRIF1 antibody detects Ligand-dependent nuclear receptor-interacting factor 1, a chromatin-associated protein that modulates gene expression through interaction with retinoblastoma protein (RB1) and nuclear receptors. The UniProt recommended name is Ligand-dependent nuclear receptor-interacting factor 1 (LRIF1). Also known as RIF-1 or HBiX1, this protein functions in transcriptional repression and chromatin organization at heterochromatic regions.\u003cbr\u003e\u003cbr\u003eFunctionally, LRIF1 antibody identifies a 568-amino-acid nuclear protein that forms complexes with RB1 and heterochromatin protein 1 (HP1). LRIF1 recruits repressor complexes to chromatin and stabilizes transcriptionally silent regions, contributing to epigenetic control of gene activity. It also enhances ligand-dependent transcriptional regulation by nuclear receptors, integrating hormonal signaling with chromatin structure.\u003cbr\u003e\u003cbr\u003eThe LRIF1 gene is located on chromosome 1p36.11 and is ubiquitously expressed in proliferating and differentiated cells. LRIF1 plays key roles in cell cycle regulation, transcriptional silencing, and heterochromatin maintenance, particularly in gene-rich nuclear domains where it supports long-term epigenetic repression.\u003cbr\u003e\u003cbr\u003ePathologically, altered LRIF1 expression or mutation has been implicated in cancer progression and neurodevelopmental disorders through dysregulated chromatin remodeling. Its interaction with RB1 links LRIF1 to tumor-suppressive pathways that control proliferation and differentiation. Research using LRIF1 antibody supports studies in epigenetics, nuclear receptor signaling, and transcriptional repression.\u003cbr\u003e\u003cbr\u003eLRIF1 antibody is validated for western blotting, immunoprecipitation, and immunofluorescence to detect chromatin-associated factors and nuclear corepressors. \u003cbr\u003e\u003cbr\u003eStructurally, Ligand-dependent nuclear receptor-interacting factor 1 contains coiled-coil and leucine-rich motifs mediating protein-protein interactions with RB1 and HP1. This antibody aids exploration of LRIF1's role in heterochromatin stability and nuclear receptor signaling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LRIF1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LRIF1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LRIF1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LRIF1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LRIF1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313498477,"sku":"FY13158","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_997c3a09-7541-46ee-9517-09825f5878da.jpg?v=1782237092","url":"https:\/\/www.ebiohippo.com\/products\/lrif1-antibody-ligand-dependent-nuclear-receptor-interacting-factor-1-rif-1-hbix1-c1orf103-bha17136060","provider":"BioHippo","version":"1.0","type":"link"}