{"product_id":"lsm14a-antibody-rap55-alphasnbp-bha17135378","title":"LSM14A Antibody \/ RAP55 \/ AlphaSNBP","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLSM14A Antibody \/ RAP55 \/ AlphaSNBP is a anti-LSM14A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm (P-body, stress granule).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LSM14A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLSM14A antibody detects Sm-like protein LSM14A, a cytoplasmic RNA-binding protein that functions in mRNA storage, decay, and translational control. LSM14A is a core component of processing bodies (P-bodies), dynamic cytoplasmic granules involved in mRNA surveillance and post-transcriptional gene regulation. It plays a key role in mRNA decapping and interacts with proteins such as DDX6, EDC3, and PATL1 to form ribonucleoprotein complexes. The LSM14A antibody is essential for studying mRNA metabolism, stress response, and the regulation of gene expression at the post-transcriptional level.\u003cbr\u003e\u003cbr\u003eLSM14A is encoded by the LSM14A gene located on human chromosome 19p13.2. The protein is approximately 46 kilodaltons and contains an Sm-like domain that mediates RNA binding and protein-protein interactions. It also includes a C-terminal FDF motif required for association with DDX6 helicase, a central regulator of mRNA decapping and translational repression. LSM14A localizes to discrete cytoplasmic foci corresponding to P-bodies, which dynamically assemble and disassemble in response to stress and cellular signaling. Through its activity, LSM14A influences mRNA stability and translation during differentiation and immune activation.\u003cbr\u003e\u003cbr\u003eThe LSM14A antibody reveals strong cytoplasmic punctate staining under conditions that promote P-body formation, such as serum starvation or oxidative stress. Western blot analysis typically detects a single band near 51 kilodaltons. Functional studies demonstrate that loss of LSM14A impairs mRNA decay, leading to prolonged expression of transiently expressed transcripts. Moreover, LSM14A participates in innate immune responses by regulating the stability of interferon-related mRNAs, linking RNA metabolism to antiviral defense mechanisms.\u003cbr\u003e\u003cbr\u003eRecent research indicates that LSM14A interacts with other RNA granule components, including GW182 and AGO2, bridging mRNA silencing and decay pathways. It also contributes to stress granule dynamics and may influence autophagy signaling through mRNA control. Dysregulation of LSM14A expression has been observed in certain cancers and neurodegenerative diseases, highlighting its importance in cellular stress adaptation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LSM14A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LSM14A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LSM14A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LSM14A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LSM14A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289381229,"sku":"FY12475","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8b25e649-0945-49fa-982f-8e9e58e153d9.jpg?v=1782237012","url":"https:\/\/www.ebiohippo.com\/products\/lsm14a-antibody-rap55-alphasnbp-bha17135378","provider":"BioHippo","version":"1.0","type":"link"}