{"product_id":"luc-akt-ph-yfp-stable-mcf-7-b2-stable-cell-line-bhc10901283","title":"Luc-Akt-PH\/YFP Stable MCF-7\/B2 Stable Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eLuc-Akt-PH\/YFP Stable MCF-7\/B2 Stable Cell Line express the pleckstrin homology (PH) domain of Akt fused to Renilla luciferase with a YFP-Mem, allowing the ability to monitor the PIP3 production in real time through the established BRET biosensor pair. In which the Luc-Akt-PH will be recruited to the plasma membrane due to PIP3 production which then results in an energy transfer between the luciferase and YFP.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Luc-Akt-PH\/YFP Stable MCF-7\/B2 Stable Cell Line is supplied as a tumor cell line derived from Human mammary.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 1.0 mg\/ml Geneticin\/G418 (G271) and 200 µg\/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Luciferase reporter expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in mammary biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe cells are suitable for the screening and study of molecules on the PI3K\/Akt pathway for diabetes and cancer-related research. T3160) for experimental studies. Donor\/background information provided for this product: Female, 69, Caucasian, Adenocarcinoma.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 1.0 mg\/ml Geneticin\/G418 (G271) and 200 µg\/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180522430829,"sku":"T3161","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/zmIkjg5HCS2p2kNmXDNCixBf2txzFIvRmjUgB5fI.png?v=1774957810","url":"https:\/\/www.ebiohippo.com\/products\/luc-akt-ph-yfp-stable-mcf-7-b2-stable-cell-line-bhc10901283","provider":"BioHippo","version":"1.0","type":"link"}