{"product_id":"lv-ef1a-spcas9-bhv22000044","title":"LV-EF1a-SpCas9","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eLentiviral Vector\u003c\/span\u003e\u003ch2\u003eVector Overview\u003c\/h2\u003e\n\u003cp\u003eLV-EF1a-SpCas9 is a pre-made lentivirus that overexpresses SpCas9 under the EF1a promoter and can be used along with guided RNA for genome editing. The Type II prokaryotic CRISPR\/Cas system is the new class of tools for targeted genome engineering. Cas9 (CRISPR-associated protein 9) is an RNA-guided DNA nuclease enzyme associated with Streptococcus pyogenes’ CRISPR immunity system. The Cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems use small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR\/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. Ready to use format.\u003c\/p\u003e\n\u003ch2\u003eTechnical Details\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog No.\u003c\/th\u003e\n\u003ctd\u003eSL100307\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePromoter\u003c\/th\u003e\n\u003ctd\u003eEF1a\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTransgene\u003c\/th\u003e\n\u003ctd\u003eSpCas9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression Regulation\u003c\/th\u003e\n\u003ctd\u003eConstitutive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSelection Marker\u003c\/th\u003e\n\u003ctd\u003eNone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTiter\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;1E+9 TU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Buffer\u003c\/th\u003e\n\u003ctd\u003ePBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity \/ Grade\u003c\/th\u003e\n\u003ctd\u003eIn vivo grade and OK for both in vitro tissue culture infection and in vivo injection * Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiosafety Level\u003c\/th\u003e\n\u003ctd\u003eBSL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e25 uL; 25 uL x 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLentiviral Transduction\u003c\/strong\u003e: stable delivery of the cassette into dividing and non-dividing cells for long-term expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGene Editing\u003c\/strong\u003e: CRISPR\/Cas9-mediated genome editing when combined with a guide RNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiosafety\u003c\/h2\u003e\n\u003cp\u003eLV-EF1a-SpCas9 is a pre-packaged lentiviral vector classified as BSL-2. Handle in a certified biosafety cabinet under institutional BSL-2 practices, decontaminate all contact surfaces and waste, and follow local regulations for viral vector work.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eStore at -80°C; avoid repeated freeze-thaw cycles. Ships on dry ice. Supplied in PBS. For research use only; not for diagnostic or therapeutic use.\u003c\/p\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"25 uL","offer_id":53987160064365,"sku":"SL100307-25UL","price":236.0,"currency_code":"USD","in_stock":true},{"title":"25 uL x 2","offer_id":53987163472237,"sku":"SL100307-25ULX2","price":468.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LV-EF1a-SpCas9.webp?v=1789660670","url":"https:\/\/www.ebiohippo.com\/products\/lv-ef1a-spcas9-bhv22000044","provider":"BioHippo","version":"1.0","type":"link"}