{"product_id":"lv-ef1a-tagrfp-puro-bhv22000090","title":"LV-EF1a-TagRFP-Puro","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eLentiviral Vector\u003c\/span\u003e\u003ch2\u003eVector Overview\u003c\/h2\u003e\n\u003cp\u003eLV-EF1a-TagRFP-Puro is a pre-packaged recombinant lentiviral vector that drives expression of TagRFP under the control of the human elongation factor 1-alpha (EF1a) promoter. The EF1a promoter is a strong, ubiquitous mammalian promoter derived from the human EF1A gene. It provides sustained, high-level expression across a wide range of cell types, including primary cells and stem cells, and is less susceptible to transgene silencing than viral promoters such as CMV. TagRFP is a bright, monomeric red-orange fluorescent protein with high quantum yield and excellent photostability. It offers strong fluorescence intensity per molecule, making it suitable for applications that require sensitive detection of low-expression events. The puromycin resistance cassette allows efficient antibiotic selection of successfully transduced cells, enabling rapid generation of stable, enriched cell populations. Lentiviral delivery ensures efficient, stable integration into the host genome of both dividing and non-dividing mammalian cells. LV-EF1a-TagRFP-Puro is supplied in a ready-to-use format, eliminating the need for vector construction, packaging, and titration.\u003c\/p\u003e\n\u003ch2\u003eTechnical Details\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog No.\u003c\/th\u003e\n\u003ctd\u003eSL100370\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePromoter\u003c\/th\u003e\n\u003ctd\u003eEF1a\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter \/ Tag\u003c\/th\u003e\n\u003ctd\u003eTagRFP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression Regulation\u003c\/th\u003e\n\u003ctd\u003eConstitutive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSelection Marker\u003c\/th\u003e\n\u003ctd\u003ePuromycin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTiter\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;1E+9 TU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Buffer\u003c\/th\u003e\n\u003ctd\u003ePBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity \/ Grade\u003c\/th\u003e\n\u003ctd\u003eIn vivo grade and OK for both in vitro tissue culture infection and in vivo injection * Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiosafety Level\u003c\/th\u003e\n\u003ctd\u003eBSL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e25 uL; 25 uL x 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eLentiviral Transduction\u003c\/strong\u003e: stable delivery of the cassette into dividing and non-dividing cells for long-term expression.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eBiosafety\u003c\/h2\u003e\n\u003cp\u003eLV-EF1a-TagRFP-Puro is a pre-packaged lentiviral vector classified as BSL-2. Handle in a certified biosafety cabinet under institutional BSL-2 practices, decontaminate all contact surfaces and waste, and follow local regulations for viral vector work.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eStore at -80°C; avoid repeated freeze-thaw cycles. Ships on dry ice. Supplied in PBS. For research use only; not for diagnostic or therapeutic use.\u003c\/p\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"25 uL","offer_id":53987161604461,"sku":"SL100370-25UL","price":236.0,"currency_code":"USD","in_stock":true},{"title":"25 uL x 2","offer_id":53987166191981,"sku":"SL100370-25ULX2","price":468.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LV-EF1a-TagRFP-Puro.webp?v=1789660672","url":"https:\/\/www.ebiohippo.com\/products\/lv-ef1a-tagrfp-puro-bhv22000090","provider":"BioHippo","version":"1.0","type":"link"}