{"product_id":"lx-2-cells-bhc10902266","title":"LX-2 Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eLX-2 is an immortalized human hepatic stellate cell (HSC) line commonly used as an in vitro model for investigating liver fibrosis, hepatic inflammation, and extracellular matrix (ECM) remodeling. These cells retain hallmark features of activated stellate cells, including robust expression of α-smooth muscle actin (α-SMA) and a functional response to profibrotic cytokines such as transforming growth factor-beta 1 (TGF-β1).LX-2 cells were derived from primary human hepatic stellate cells and immortalized via stable expression of the Simian Virus 40 (SV40) large T antigen, enabling sustained proliferation while preserving key fibrogenic characteristics.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e LX-2 Cells is supplied as an immortalized cell line derived from Human liver.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. To thaw the cells, use Thawing growth media: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Once cells are attached and recovered, change to Maintenance growth media: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 2% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate Note: Do not culture LX‑2 cells long-term in 10% FBS; use 2% FBS for routine maintenance to preserve a quiescent-like phenotype and prevent activation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in vascular biology, signaling, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in vascular biology, signaling, and assay development. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Male.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. To thaw the cells, use Thawing growth media: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Once cells are attached and recovered, change to Maintenance growth media: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 2% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate Note: Do not culture LX‑2 cells long-term in 10% FBS; use 2% FBS for routine maintenance to preserve a quiescent-like phenotype and prevent activation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2-1:3\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180545171821,"sku":"T1924","price":329.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/gfqYpMS6nLZ5LRe9VqmWSezpEABdVYh92uYzieBV.png?v=1782151806","url":"https:\/\/www.ebiohippo.com\/products\/lx-2-cells-bhc10902266","provider":"BioHippo","version":"1.0","type":"link"}