{"product_id":"lyar-antibody-cell-growth-regulating-nucleolar-protein-bha17135296","title":"LYAR Antibody \/ Cell growth-regulating nucleolar protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eLYAR Antibody \/ Cell growth-regulating nucleolar protein is a anti-LYAR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleolus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LYAR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe LYAR antibody targets Cell growth-regulating nucleolar protein, a zinc finger nucleolar protein encoded by the LYAR gene. Originally identified in human leukemia cells, LYAR plays roles in ribosome biogenesis, rRNA processing, and cell proliferation. Cell growth-regulating nucleolar protein associates with pre-ribosomal complexes and contributes to ribosomal subunit maturation and export. The LYAR antibody provides an essential tool for investigating nucleolar function, ribosome assembly, and growth regulation in both normal and transformed cells.\u003cbr\u003e\u003cbr\u003eCell growth-regulating nucleolar protein contains a C2HC-type zinc finger motif and a nuclear localization signal, directing it to the nucleolus where ribosome synthesis occurs. It participates in the early stages of 60S ribosomal subunit formation by binding pre-rRNA and ribonucleoprotein complexes. The LYAR antibody enables detection of this nucleolar marker, supporting studies into the molecular events that control ribosome production and cellular growth rates. Its expression correlates with proliferative activity in rapidly dividing cells.\u003cbr\u003e\u003cbr\u003eLYAR has emerged as a multifunctional protein involved in transcriptional regulation and cell cycle progression. It interacts with nucleolin and other nucleolar proteins to coordinate ribosomal RNA processing. Beyond ribosome synthesis, Cell growth-regulating nucleolar protein influences p53 signaling and apoptosis by modulating nucleolar stress responses. The LYAR antibody supports analysis of these pathways, helping define how nucleolar integrity links to cell cycle checkpoints and stress-induced growth arrest.\u003cbr\u003e\u003cbr\u003eOverexpression of LYAR has been observed in several cancers, including colorectal, prostate, and hepatocellular carcinoma. It promotes cell proliferation and survival by enhancing rRNA synthesis and suppressing apoptosis. The LYAR antibody enables quantitative assessment of its expression in tumor tissues and cell models, aiding investigation into oncogenic mechanisms involving ribosome biogenesis. In contrast, depletion of LYAR impairs ribosomal maturation and reduces cell growth, underscoring its importance for proliferation control.\u003cbr\u003e\u003cbr\u003eThe LYAR antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing distinct nucleolar staining consistent with its localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LYAR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LYAR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LYAR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LYAR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LYAR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287087469,"sku":"FY12393","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ba3230e1-33be-4656-a548-97998cff3157.jpg?v=1782236992","url":"https:\/\/www.ebiohippo.com\/products\/lyar-antibody-cell-growth-regulating-nucleolar-protein-bha17135296","provider":"BioHippo","version":"1.0","type":"link"}