{"product_id":"lymph-node-metastasis-melanoma-cell-line-mm057-bhc10902008","title":"Lymph Node Metastasis Melanoma Cell Line (MM057)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis melanoma cell line panel is unique from all other available melanoma cell lines as these cells have been raised under conditions that prevent phenotype switching.. MM057 is applicable for high-throughput screening\/Drug testing and 3D cell cultures.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Lymph Node Metastasis Melanoma Cell Line (MM057) is supplied as a tumor cell line derived from Human lymph node.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, melanocytic state with increased mesenchymal-like properties\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F10 Medium + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cancer biology, phenotype comparison, and response profiling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis cell line displays intermediate sensitivity to MEKi. Additional cell lines from this panel include:Cat. T8080 - Acral Lentiginous Melanoma Cell Line (HBL (MM001)) Cat. T8081 - Acral Lentiginous Melanoma Cell Line (MM011\/LND1) Cat. T8082 - Lymph Node Metastasis SS Melanoma Cell Line (MM029) Cat. T8083 - Bladder Metastasis Nodular Melanoma Cell Line (MM031) Cat. T8084 - Lymph Node Metastasis Melanoma Cell Line (MM034) Cat. T8085 - Superficial Spreading Melanoma Cell Line (MM052) Cat. T8086 - Lymph Node Metastasis Melanoma Cell Line (MM057) Cat. T8087 - Lymph Node Metastasis SS Melanoma Cell Line (MM074) Cat. T8088 - Lymph Node Metastasis SS Melanoma Cell Line (MM165) Donor\/background information provided for this product: Derived from a lymph node metastasis.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F10 Medium + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2 to 1:5\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180538061165,"sku":"T8086","price":607.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/RedsUpu0dBBogTXtCPGKmQuW7CiXCk9iqq6TmvpY.png?v=1774957849","url":"https:\/\/www.ebiohippo.com\/products\/lymph-node-metastasis-melanoma-cell-line-mm057-bhc10902008","provider":"BioHippo","version":"1.0","type":"link"}