{"product_id":"lymph-node-metastasis-ss-melanoma-cell-line-mm029-bhc10902010","title":"Lymph Node Metastasis SS Melanoma Cell Line (MM029)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis melanoma cell line panel is unique from all other available melanoma cell lines as these cells have been raised under conditions that prevent phenotype switching. Derived from a lymph node metastasis of a female with superficial spreading melanoma (SSM), MM029 has intrinsic resistance to BRAF inhibitor.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Lymph Node Metastasis SS Melanoma Cell Line (MM029) is supplied as a tumor cell line derived from Human lymph node.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, invasive\/mesenchymal like phenotype\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F10 Medium + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cancer biology, phenotype comparison, and response profiling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis cell line is applicable for high-throughput screening\/drug testing and 3D cell culture. Note: This cell line should be cited in a scientific publication as: MM029. Additional cell lines from this panel include:Cat. T8080 - Acral Lentiginous Melanoma Cell Line (HBL (MM001)) Cat. T8081 - Acral Lentiginous Melanoma Cell Line (MM011\/LND1) Cat. T8082 - Lymph Node Metastasis SS Melanoma Cell Line (MM029) Cat. T8083 - Bladder Metastasis Nodular Melanoma Cell Line (MM031) Cat. T8084 - Lymph Node Metastasis Melanoma Cell Line (MM034) Cat. T8085 - Superficial Spreading Melanoma Cell Line (MM052) Cat. T8086 - Lymph Node Metastasis Melanoma Cell Line (MM057) Cat. T8087 - Lymph Node Metastasis SS Melanoma Cell Line (MM074) Cat. T8088 - Lymph Node Metastasis SS Melanoma Cell Line (MM165) Donor\/background information provided for this product: Derived from a lymph node metastasis of a female with superficial spreading melanoma (SSM).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F10 Medium + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180538028397,"sku":"T8082","price":607.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/jrk8r0S4jA3zemKJQdyBj2kVoWfq80pDV2bunmcj.png?v=1774957848","url":"https:\/\/www.ebiohippo.com\/products\/lymph-node-metastasis-ss-melanoma-cell-line-mm029-bhc10902010","provider":"BioHippo","version":"1.0","type":"link"}