{"product_id":"lyophilized-bst-plus-dna-polymerase-60-u-mu-l-glycerol-free-bhz20800085","title":"Lyophilized Bst Plus DNA Polymerase (60 U\/μL, Glycerol-Free)","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eHieff® Bst Plus DNA Polymerase is derived from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eBacillus stearothermophilus\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eDNA Polymerase I. The 5 '-3' exonuclease activity was removed by genetic engineering method, but the 5 '-3' DNA polymerase activity and strong chain replacement activity were retained. Compared with wild-type Bst DNA Polymerase, the polymerase showed great improvement in amplification speed, yield, salt tolerance and thermal stability, and increased dUTP tolerance. It was very suitable for isothermal amplification reactions such as LAMP reaction. This product freeze-dried glycerin-free Bst Plus DNA Polymerase to solve the problem of glycerinase-free instability and could be used to make freeze-dried products.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eHigh amplification efficiency\u003c\/li\u003e\n\u003cli\u003eHigh dUTP tolerance\u003c\/li\u003e\n\u003cli\u003eThe freeze-dried product form\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eIsothermal DNA amplification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePolymerase\u003c\/td\u003e\n\u003ctd\u003eBst DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeat Inactivation\u003c\/td\u003e\n\u003ctd\u003eIncubation at 85°C for 5 min.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eActivity Definition\u003c\/td\u003e\n\u003ctd\u003e1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e14405ES60 (12 KU)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e14405ES97 (120 KU)\u003c\/td\u003e\n\u003ctd\u003e14402ES98 (1,200 KU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e14405-A\u003c\/td\u003e\n\u003ctd\u003eLyophilized Bst Plus DNA Polymerase (60 U\/μL，Glycerol-Free)\u003c\/td\u003e\n\u003ctd\u003e1 Vial（200 μL）\u003c\/td\u003e\n\u003ctd\u003e5 Vials(400 μL)\u003c\/td\u003e\n\u003ctd\u003e10 Vials(2 mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e14405-B\u003c\/td\u003e\n\u003ctd\u003eLyophilized monoenzyme redissolution solution\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e2 mL\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003e\u003cspan\u003eHigh Sensitivity\u003c\/span\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/384681\/131a-167524194263128440.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 1: Sensitivity Test\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRed: 50 copies\/T, response less than 15 minutes\u003cbr\u003eFigure 1: The copy numbers of 50 copies\/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies\/T performed better, and the detection rate reached 100%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003e\u003cspan\u003eStability\u003c\/span\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/93756\/3580-167524288433759822.jpg\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 2.Stability testing at the same temperature\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRed: 37℃ 0 day  Blue: 37℃ 1 day  Green: 37℃ 3 days  Purple: 37℃ 5 days\u003cbr\u003e0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/384681\/95bb-167524291024348489.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 3. -20℃ repeated freeze-thaw stability test\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRed: Repeat freeze-thaw 0 times\u003cbr\u003eBlue: Repeat freeze-thaw 5 times\u003cbr\u003eGreen: Repeat freeze-thaw 10 times\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/713959\/894b-167524318728957537.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 4. 48-well NTC-\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"12 KU","offer_id":53294148354413,"sku":"14405ES60","price":225.0,"currency_code":"USD","in_stock":true},{"title":"120 KU","offer_id":53294159495533,"sku":"14405ES97","price":2105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/131a-167524194263128440_8aae251f-0c63-4fa9-bf6f-535768352375.png?v=1778101065","url":"https:\/\/www.ebiohippo.com\/products\/lyophilized-bst-plus-dna-polymerase-60-u-mu-l-glycerol-free-bhz20800085","provider":"BioHippo","version":"1.0","type":"link"}