{"product_id":"magbead-pathogenic-microbiome-dna-rna-kit-bht15200023","title":"Magbead Pathogenic Microbiome DNA\/RNA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eNucleic Acid Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit is designed for purifying and enriching DNA and RNA from pathogenic microorganisms, such as viruses, bacteria, and fungi, present in biological fluid samples like sputum, bronchoalveolar lavage fluid, blood, and ascites. The purified microbial DNA and RNA are suitable for a variety of downstream applications, including PCR, RT-PCR, qPCR, NGS, and other molecular biology experiments.\u003c\/p\u003e\n\u003cp\u003eThe protocol mixes 400 µL of sample with 200 µL of Buffer LBS-A and 20 µL of Proteinase K, vortexed for 10 min or thermomixed at 65°C and 2500 rpm for 10 min. A second digestion with 250 µL of Buffer ML and 20 µL of Proteinase K runs at 56°C for 10 min, followed by clarification at 12,000 rpm for 1 min. Nucleic acids are bound with 400 µL of isopropanol and 30 µL of Magbeads PN at 1500 rpm for 3 min, washed twice with 800 µL of Buffer GW1 and twice with 800 µL of Buffer GW2, air-dried about 3 min and eluted in 70 µL of RNase-Free Water. For cultured samples the capacity is 1×10⁹ bacterial or fungal cells or fewer.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eMagnetic beads (Magbeads PN) with Lysis Tubes II\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eBiological fluids including sputum, bronchoalveolar lavage fluid, blood and ascites\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e400 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input (cultures)\u003c\/th\u003e\n\u003ctd\u003e≤ 1×10⁹ bacterial or fungal cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNucleic acids recovered\u003c\/th\u003e\n\u003ctd\u003eDNA and RNA of viruses, bacteria and fungi\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e70 µL RNase-Free Water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e96 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer LBS-A\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer ML\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GW1\u003c\/td\u003e\n\u003ctd\u003e180 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GW2\u003c\/td\u003e\n\u003ctd\u003e180 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free Water\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd\u003e4 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagbeads PN\u003c\/td\u003e\n\u003ctd\u003e2 × 1.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLysis Tubes II\u003c\/td\u003e\n\u003ctd\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePurification and enrichment of microbial DNA and RNA from biological fluids\u003c\/li\u003e\n\u003cli\u003eNucleic acid extraction from sputum and bronchoalveolar lavage fluid\u003c\/li\u003e\n\u003cli\u003eNucleic acid extraction from blood and ascites\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for PCR, RT-PCR and qPCR\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for NGS\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at room temperature (10–30°C) with a shelf life of 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eInspect the buffers for precipitate and dissolve them at 56°C if needed.\u003c\/li\u003e\n\u003cli\u003eStore samples at 2–8°C for the short term or at -20°C or -80°C for the long term.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze-thaw cycles of the sample.\u003c\/li\u003e\n\u003cli\u003eMaintain a clean work area and use DNA\/RNA-free tips.\u003c\/li\u003e\n\u003cli\u003eImplement contamination controls throughout the procedure.\u003c\/li\u003e\n\u003cli\u003eIsopropanol is required at the binding step and is not supplied.\u003c\/li\u003e\n\u003cli\u003eStore the eluted nucleic acid at -20°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP027\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200023\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":53965511917933,"sku":"EP027-96T","price":650.0,"currency_code":"USD","in_stock":true},{"title":"96 T x 10","offer_id":53965515260269,"sku":"EP027-96TX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP027-a-1.jpg?v=1789419111","url":"https:\/\/www.ebiohippo.com\/products\/magbead-pathogenic-microbiome-dna-rna-kit-bht15200023","provider":"BioHippo","version":"1.0","type":"link"}