{"product_id":"magbead-plant-dna-kit-bht15200020","title":"Magbead Plant DNA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit provides a simple and efficient plant DNA extraction solution. DNA in the sample binds to the surface of silica-coated magnetic beads in the presence of high salt. After rinsing, the DNA is eluted in Buffer TB or deionized water. The extraction rate is closely related to the sample type and the cell disruption effect. The extracted DNA can be used for next-generation sequencing, PCR test and other downstream experiments.\u003c\/p\u003e\n\u003cp\u003eThe protocol processes 50–100 mg of fresh material or about 30 mg of dry material. The sample is lysed in 400 µL of Buffer PLS with 5 µL of RNase A at 70°C and 1600 rpm for 10 min, clarified at 12,000 rpm for 4 min, then bound with 550 µL of Buffer ZB and 20 µL of Magbeads for 5 min. The beads are washed twice with 650 µL of Buffer GW1 and twice with 650 µL of 75% ethanol, air-dried for 5–10 minutes and eluted in 100 µL of Buffer TB at 65°C for 10 min.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSilica-coated magnetic beads (Magbeads PN)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003ePlant material\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e50–100 mg fresh material or about 30 mg dry material\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinding chemistry\u003c\/th\u003e\n\u003ctd\u003eSilica-coated magnetic beads, high-salt binding\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial treatment\u003c\/th\u003e\n\u003ctd\u003eRNase A (10 mg\/mL) supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e100 µL Buffer TB at 65°C for 10 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e96 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PLS\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer ZB\u003c\/td\u003e\n\u003ctd\u003e55 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GW1 (concentrate)\u003c\/td\u003e\n\u003ctd\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer TB\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase A (10 mg\/mL)\u003c\/td\u003e\n\u003ctd\u003e0.6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagbeads PN\u003c\/td\u003e\n\u003ctd\u003e2 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDNA extraction from fresh or dry plant material\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for next-generation sequencing\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for PCR testing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at room temperature (15–30°C) with a shelf life of 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMagbeads PN must not be frozen or subjected to high-speed centrifugation.\u003c\/li\u003e\n\u003cli\u003eFor plants rich in polysaccharides and polyphenols, β-mercaptoethanol is added to 5%; it is not supplied with the kit. See the manufacturer's manual for the buffer to which it is added.\u003c\/li\u003e\n\u003cli\u003ePrepare the 75% ethanol wash separately, as it is not supplied.\u003c\/li\u003e\n\u003cli\u003eThe extraction rate depends on the sample type and the cell disruption effect.\u003c\/li\u003e\n\u003cli\u003eStore the eluted DNA at -20°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP024\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200020\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":53965511885165,"sku":"EP024-96T","price":442.0,"currency_code":"USD","in_stock":true},{"title":"96 T x 10","offer_id":53965513982317,"sku":"EP024-96TX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP024-a-1.jpg?v=1789419111","url":"https:\/\/www.ebiohippo.com\/products\/magbead-plant-dna-kit-bht15200020","provider":"BioHippo","version":"1.0","type":"link"}