{"product_id":"magbead-plant-rna-kit-bht15200021","title":"Magbead Plant RNA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit provides a simple and efficient automated plant RNA extraction solution. The RNA in the lysate binds to the surface of silica-coated magnetic beads in the presence of high salt, and is then washed with a rinse solution to remove impurities such as proteins. DNase I is then added to remove residual DNA, and the RNA is finally washed and eluted in RNase-Free Water. The total RNA obtained is of high purity, free of contamination from genomic DNA, proteins and other impurities, and can be used for Real Time RT-PCR, RT-PCR, Northern Blot, Dot Blot and in vitro translation and other downstream experiments.\u003c\/p\u003e\n\u003cp\u003eThe protocol processes 30–100 mg of fresh or frozen plant tissue. Tissue is ground to a powder under liquid nitrogen, lysed in 600 µL of Buffer PRL and clarified at 14,000 rpm and 4°C for 5 min. RNA is bound with 350 µL of Buffer PA and 20 µL of Magbeads PN at 1700 rpm for 5 min, washed with 500 µL of Buffer PGW1, treated with 80 µL of DNase I working solution for 5 min, washed twice with 700 µL of Buffer RW2, air-dried for 5–10 min and eluted in 100 µL of RNase-Free Water at 65°C and 1700 rpm for 10 min.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSilica-coated magnetic beads (Magbeads PN)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eWorkflow\u003c\/th\u003e\n\u003ctd\u003eSuitable for automated extraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003eFresh or frozen plant tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e30–100 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial treatment\u003c\/th\u003e\n\u003ctd\u003eDNase I (1 U\/µL) with 10× Reaction Buffer supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e100 µL RNase-Free Water at 65°C, 1700 rpm, 10 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e96 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDNase I (1 U\/µL)\u003c\/td\u003e\n\u003ctd\u003e2 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10× Reaction Buffer\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PRL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PA\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PGW1 (concentrate)\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW2 (concentrate)\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free Water\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagbeads PN\u003c\/td\u003e\n\u003ctd\u003e2 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated total RNA extraction from plant tissue\u003c\/li\u003e\n\u003cli\u003eReal Time RT-PCR and RT-PCR\u003c\/li\u003e\n\u003cli\u003eNorthern Blot and Dot Blot analysis\u003c\/li\u003e\n\u003cli\u003eIn vitro translation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eDNase I and the 10× Reaction Buffer are stored at -20°C; the other components are stored at room temperature (15–30°C). The shelf life is 1 year at room temperature.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse RNase-free materials and disposable gloves.\u003c\/li\u003e\n\u003cli\u003eAvoid high-starch plant tissues, which produce gelatinous substances.\u003c\/li\u003e\n\u003cli\u003eUse fresh or liquid nitrogen-frozen samples and avoid repeated freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003ePrepare the DNase I mix fresh from 52 µL RNase-Free Water, 8 µL of buffer and 20 µL of DNase I.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP025\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200021\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":53965512245613,"sku":"EP025-96T","price":468.0,"currency_code":"USD","in_stock":true},{"title":"96 T x 10","offer_id":53965517291885,"sku":"EP025-96TX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP025-a-1.jpg?v=1789419193","url":"https:\/\/www.ebiohippo.com\/products\/magbead-plant-rna-kit-bht15200021","provider":"BioHippo","version":"1.0","type":"link"}