{"product_id":"maml1-antibody-mastermind-like-protein-1-bha17135668","title":"MAML1 Antibody \/ Mastermind-like protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMAML1 Antibody \/ Mastermind-like protein 1 is a anti-MAML1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MAML1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMAML1 antibody detects Mastermind-like protein 1, a transcriptional coactivator that plays a key role in the Notch signaling pathway, which regulates cell fate determination, differentiation, and proliferation. Encoded by the MAML1 gene on chromosome 5q35.3, this nuclear protein acts as a core component of the Notch transcriptional activation complex. Upon Notch receptor activation and nuclear translocation of the Notch intracellular domain (NICD), MAML1 binds NICD and the transcription factor CSL (CBF1\/RBPJ), forming a ternary complex that recruits additional coactivators to initiate target gene transcription.\u003cbr\u003e\u003cbr\u003eMAML1 contains an N-terminal basic domain responsible for binding NICD and CSL, a central acidic transcriptional activation domain, and a C-terminal domain that mediates interactions with additional transcription factors and coactivators such as p300\/CBP. Through these interactions, MAML1 promotes transcription of Notch target genes including HES1, HEY1, and MYC, which are involved in cell differentiation and proliferation. Because Notch signaling is crucial for tissue development and homeostasis, MAML1 serves as a master regulator of developmental pathways across multiple cell types, including neural, hematopoietic, and epithelial lineages.\u003cbr\u003e\u003cbr\u003eThe MAML1 antibody is widely used in molecular biology, developmental biology, and cancer research to assess Notch pathway activation and transcriptional regulation. Western blot analysis typically identifies a 115 kilodalton band corresponding to MAML1, while immunofluorescence reveals nuclear localization that overlaps with Notch pathway components. This antibody enables the study of transcriptional coactivation events and gene regulation in Notch-mediated signaling contexts.\u003cbr\u003e\u003cbr\u003eBeyond Notch signaling, MAML1 also functions as a coactivator for other transcriptional regulators such as MEF2C, p53, and beta-catenin, broadening its impact on gene networks related to cell growth and apoptosis. Dysregulation of MAML1 has been linked to oncogenic transformation, particularly in leukemia, glioma, and breast cancer, where aberrant Notch signaling drives uncontrolled proliferation. The MAML1 antibody provides a reliable tool for investigating transcriptional complexes and signaling crosstalk mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MAML1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MAML1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MAML1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MAML1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MAML1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299965293,"sku":"FY12765","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_db57cdd1-f23f-44c4-82b3-86b3fadc0714.jpg?v=1772019341","url":"https:\/\/www.ebiohippo.com\/products\/maml1-antibody-mastermind-like-protein-1-bha17135668","provider":"BioHippo","version":"1.0","type":"link"}