{"product_id":"maml3-antibody-mastermind-like-protein-3-bha17135277","title":"MAML3 Antibody \/ Mastermind-like protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMAML3 Antibody \/ Mastermind-like protein 3 is a anti-MAML3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear speckles.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MAML3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe MAML3 antibody targets Mastermind-like protein 3, a transcriptional coactivator encoded by the MAML3 gene. This protein is a member of the Mastermind-like family, which serves as a key nuclear coactivator in the Notch signaling pathway. Mastermind-like protein 3 interacts with the intracellular domain of Notch receptors and the transcription factor CSL (CBF1\/RBPJ) to assemble transcriptional activation complexes that regulate cell fate decisions, proliferation, and differentiation. The MAML3 antibody provides researchers with a powerful tool for studying Notch-dependent transcriptional control and its roles in development and oncogenesis.\u003cbr\u003e\u003cbr\u003eMastermind-like protein 3 acts as a scaffold that stabilizes the Notch-CSL complex on target promoters, recruiting additional coactivators such as p300\/CBP to initiate transcription. Its activation leads to expression of Notch target genes, including those involved in stem cell maintenance, neural development, and tumorigenesis. The MAML3 antibody supports studies aimed at elucidating these transcriptional networks by allowing detection and localization of MAML3 in nuclear extracts and tissue sections.\u003cbr\u003e\u003cbr\u003eChromosomal rearrangements involving MAML3 have been implicated in human cancers, notably biphenotypic sinonasal sarcoma and certain neuroendocrine tumors. The resulting fusion proteins, such as PAX3-MAML3, act as potent transcriptional activators driving oncogenic programs. The MAML3 antibody enables researchers to analyze wild-type and fusion variants, supporting investigations into the molecular mechanisms by which Mastermind-like protein 3 contributes to tumor development and aberrant Notch signaling.\u003cbr\u003e\u003cbr\u003eBeyond its canonical Notch-related functions, Mastermind-like protein 3 participates in other signaling pathways, including Wnt and Hedgehog, where it modulates transcriptional responses through interactions with diverse transcription factors. The MAML3 antibody aids in identifying these alternative regulatory roles, helping to map MAML3-containing transcriptional complexes in various tissues and developmental stages. Expression analysis indicates enrichment of MAML3 in neural and mesenchymal lineages, consistent with its role in differentiation control.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MAML3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MAML3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MAML3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MAML3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MAML3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286661485,"sku":"FY12374","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5e502e82-572e-452d-be38-74a9dab866d9.jpg?v=1782237010","url":"https:\/\/www.ebiohippo.com\/products\/maml3-antibody-mastermind-like-protein-3-bha17135277","provider":"BioHippo","version":"1.0","type":"link"}