{"product_id":"man2c1-antibody-mannosidase-alpha-class-2c-member-1-bha17135244","title":"MAN2C1 Antibody \/ Mannosidase alpha class 2C member 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMAN2C1 Antibody \/ Mannosidase alpha class 2C member 1 is a anti-MAN2C1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MAN2C1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe MAN2C1 antibody targets Mannosidase alpha class 2C member 1, an essential enzyme in the catabolic pathway of N-linked glycoproteins. Encoded by the MAN2C1 gene, this cytosolic alpha-mannosidase hydrolyzes terminal mannose residues from oligosaccharides generated during glycoprotein degradation. Through this process, Mannosidase alpha class 2C member 1 participates in the recycling of glycan intermediates and maintenance of carbohydrate homeostasis within cells. The MAN2C1 antibody enables researchers to study this crucial enzyme's distribution, enzymatic regulation, and contribution to cellular metabolism.\u003cbr\u003e\u003cbr\u003eMannosidase alpha class 2C member 1 is expressed in a wide range of tissues, including liver, kidney, and brain, where it contributes to the breakdown of misfolded glycoproteins following endoplasmic reticulum�associated degradation (ERAD). As improperly folded glycoproteins are retro-translocated to the cytosol for proteasomal degradation, MAN2C1 helps trim mannose residues from liberated oligosaccharides. This function is key for maintaining protein quality control and preventing toxic accumulation of misprocessed glycans. The MAN2C1 antibody is frequently employed in studies focused on carbohydrate metabolism, glycoprotein turnover, and the molecular mechanisms that connect glycan processing with proteostasis.\u003cbr\u003e\u003cbr\u003eIn addition to its metabolic role, Mannosidase alpha class 2C member 1 has been implicated in cell signaling and tumor biology. Evidence suggests that overexpression of MAN2C1 may suppress PTEN activity and promote oncogenic signaling through the AKT pathway. Conversely, reduced enzyme expression can influence apoptosis sensitivity and cell-cycle regulation. These findings highlight the enzyme's dual role as both a metabolic and signaling regulator. The MAN2C1 antibody allows scientists to quantify these expression changes and assess protein�protein interactions that link glycan metabolism to oncogenesis.\u003cbr\u003e\u003cbr\u003eExperimental use of the MAN2C1 antibody includes western blotting for protein detection, immunofluorescence for subcellular localization, and immunohistochemistry for tissue-level expression mapping. The enzyme is mainly cytoplasmic but may associate with endoplasmic reticulum membranes under certain physiological conditions. Researchers utilize the MAN2C1 antibody to characterize these distribution patterns and examine how post-translational modifications affect enzyme stability and activity.\u003cbr\u003e\u003cbr\u003eBecause glycoprotein metabolism influences immune recognition and cellular signaling, Mannosidase alpha class 2C member 1 has become an attractive research target across immunology, cancer, and metabolic disease. The MAN2C1 antibody provided by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MAN2C1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MAN2C1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MAN2C1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MAN2C1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MAN2C1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284859245,"sku":"FY12341","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7bcffb0a-0172-4709-8dc3-b2ddba5f2fe0.jpg?v=1782236984","url":"https:\/\/www.ebiohippo.com\/products\/man2c1-antibody-mannosidase-alpha-class-2c-member-1-bha17135244","provider":"BioHippo","version":"1.0","type":"link"}