{"product_id":"map2k1-antibody-mitogen-activated-protein-kinase-kinase-1-bha17135537","title":"MAP2K1 Antibody \/ Mitogen-activated protein kinase kinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMAP2K1 Antibody \/ Mitogen-activated protein kinase kinase 1 is a anti-MAP2K1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MAP2K1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMAP2K1 antibody detects Mitogen-activated protein kinase kinase 1, a dual-specificity protein kinase that functions as a central component of the MAPK\/ERK signaling pathway. MAP2K1, also known as MEK1, phosphorylates and activates ERK1 and ERK2, controlling key cellular processes including proliferation, differentiation, and survival. The MAP2K1 antibody is widely used in signal transduction, oncology, and developmental biology to study MAPK pathway activation and kinase regulation.\u003cbr\u003e\u003cbr\u003eMAP2K1 is encoded by the MAP2K1 gene located on human chromosome 15q22.31. The protein is approximately 393 amino acids in length and consists of an N-terminal regulatory region and a C-terminal kinase domain. MAP2K1 belongs to the family of dual-specificity kinases, capable of phosphorylating both threonine and tyrosine residues on its substrates. Activation occurs through phosphorylation by RAF kinases in response to growth factors, hormones, or stress stimuli.\u003cbr\u003e\u003cbr\u003eThe MAP2K1 antibody detects a 45 kilodalton protein by western blot and reveals cytoplasmic and nuclear localization under immunofluorescence microscopy. In resting cells, MAP2K1 is inactive; upon stimulation of RAS-RAF signaling, it becomes phosphorylated at serine residues 218 and 222, enabling catalytic activation of ERK kinases. This cascade transmits extracellular signals to transcription factors, leading to gene expression changes that drive cell proliferation and differentiation.\u003cbr\u003e\u003cbr\u003eAberrant MAP2K1 activation is implicated in numerous cancers, including melanoma, colorectal, and lung carcinoma, due to constitutive activation of the MAPK pathway. Mutations that enhance MAP2K1 activity or confer resistance to RAF inhibitors are clinically significant in targeted cancer therapy. Beyond oncology, MAP2K1 contributes to cardiac hypertrophy, immune signaling, and neural plasticity.\u003cbr\u003e\u003cbr\u003eBecause of its central role in MAPK pathway regulation, MAP2K1 serves as a key target for understanding cell signaling and therapeutic intervention.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MAP2K1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MAP2K1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MAP2K1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MAP2K1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MAP2K1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294656877,"sku":"FY12634","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f1681860-01c8-41d1-b9cd-a5bd0ef2b68a.jpg?v=1782237028","url":"https:\/\/www.ebiohippo.com\/products\/map2k1-antibody-mitogen-activated-protein-kinase-kinase-1-bha17135537","provider":"BioHippo","version":"1.0","type":"link"}