{"product_id":"mape-antibody-prame-bha17128168","title":"MAPE Antibody \/ PRAME","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eMAPE Antibody \/ PRAME is an antibody targeting \u003cstrong\u003ePRAME\u003c\/strong\u003e, raised in \u003cstrong\u003eRabbit\u003c\/strong\u003e for protein detection and localization studies where these specifications are required.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRAME (reported localization: Cytoplasm, Nucleus, Cell Membrane).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); Rabbit Ig.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Unconjugated (affects detection chemistry and multiplex compatibility).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eListed applications:\u003c\/strong\u003e WB, IHC-P, FACS (refer to on-page specifications for application-specific guidance).\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eMAPE (Melanoma antigen preferentially expressed in tumors), also called PRAME (Preferentially expressed antigen of melanoma), is a protein that is preferentially expressed in tumors, particularly in melanoma. It plays a vital role in regulating cell proliferation, migration, and invasion, making it a promising target for therapeutic interventions. Studies have shown that the overexpression of MAPE protein is associated with aggressive melanoma subtypes, indicating its potential as a prognostic biomarker in melanoma patients.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eComparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).\u003c\/li\u003e \u003cli\u003eSubcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.\u003c\/li\u003e \u003cli\u003eIntegration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eImmunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eFlow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands\/signals accordingly.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eEpitope context:\u003c\/strong\u003e binding can depend on protein conformation and sample processing; region information in the title\/immunogen can help anticipate what may be detected.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies differences:\u003c\/strong\u003e predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include negative controls (no-primary\/isotype), and where possible genetic controls (KO\/KD) or independent antibodies to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry P78395 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P78395 - Gene search: PRAME — NCBI Gene — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRAME - Ensembl search: PRAME — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRAME - PubMed search: PRAME antibody — PubMed — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRAME+antibody - Reactome search: PRAME — Reactome — https:\/\/reactome.org\/content\/query?q=PRAME --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.08 ml","offer_id":53046411886957,"sku":"F55105-0.08ML","price":205.0,"currency_code":"USD","in_stock":true},{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.4 ml","offer_id":53046469689709,"sku":"F55105-0.4ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ee40884d-b678-459e-a742-5c3f0582acf6.jpg?v=1772000495","url":"https:\/\/www.ebiohippo.com\/products\/mape-antibody-prame-bha17128168","provider":"BioHippo","version":"1.0","type":"link"}