{"product_id":"mapk14-antibody-mitogen-activated-protein-kinase-14-p38-alpha-bha17135665","title":"MAPK14 Antibody \/ Mitogen-activated protein kinase 14 \/ p38 alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMAPK14 Antibody \/ Mitogen-activated protein kinase 14 \/ p38 alpha is a anti-MAPK14 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Flow cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MAPK14\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e FACS, IP, IHC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMAPK14 antibody detects Mitogen-activated protein kinase 14 (also known as p38 alpha), a serine\/threonine kinase that plays a pivotal role in cellular responses to stress stimuli and proinflammatory cytokines. Encoded by the MAPK14 gene on chromosome 6p21.31, this kinase is part of the MAP kinase family, which transduces extracellular signals into transcriptional and post-translational responses controlling cell proliferation, differentiation, and survival. p38 alpha is activated by environmental stressors such as UV irradiation, heat shock, osmotic stress, and inflammatory signals, leading to the phosphorylation of numerous substrates that regulate gene expression and apoptosis.\u003cbr\u003e\u003cbr\u003eMAPK14 is activated by upstream kinases MKK3 and MKK6 through dual phosphorylation of the conserved threonine-glycine-tyrosine (TGY) motif in its activation loop. Once activated, p38 alpha phosphorylates a wide array of targets including transcription factors (ATF2, MEF2C, ELK1), kinases (MAPKAPK2\/3), and cell-cycle regulators, influencing inflammatory cytokine production, cell differentiation, and immune activation. It also regulates mRNA stability through the phosphorylation of RNA-binding proteins. In immune cells, p38 alpha drives the production of interleukin-6 and tumor necrosis factor-alpha, linking it to inflammatory diseases and autoimmune pathogenesis.\u003cbr\u003e\u003cbr\u003eThe MAPK14 antibody is widely used in molecular biology, inflammation, and cancer research to assess kinase expression, activation, and signal transduction. Western blot analysis typically identifies a 43 kilodalton band corresponding to MAPK14, while immunofluorescence and immunohistochemistry reveal cytoplasmic and nuclear staining depending on activation state. This antibody allows for monitoring of stress kinase activation and signaling pathway dynamics in diverse cell types.\u003cbr\u003e\u003cbr\u003eMAPK14 is also implicated in tumor biology, as it influences cell migration, angiogenesis, and resistance to chemotherapy. Its inhibition has therapeutic potential in cancer, arthritis, and neuroinflammation. The MAPK14 antibody provides a reliable tool for studying stress-activated kinase pathways, cytokine regulation, and drug mechanism of action.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MAPK14 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MAPK14 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MAPK14 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MAPK14 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MAPK14+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299506541,"sku":"FY12762","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9fa1e1af-51c6-42a5-8cb8-4f0d4ac9f681.jpg?v=1782237057","url":"https:\/\/www.ebiohippo.com\/products\/mapk14-antibody-mitogen-activated-protein-kinase-14-p38-alpha-bha17135665","provider":"BioHippo","version":"1.0","type":"link"}