{"product_id":"marcks-antibody-myristoylated-alanine-rich-c-kinase-substrate-bha17135382","title":"MARCKS Antibody \/ Myristoylated alanine-rich C-kinase substrate","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMARCKS Antibody \/ Myristoylated alanine-rich C-kinase substrate is a anti-MARCKS Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MARCKS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMARCKS antibody detects Myristoylated alanine-rich C-kinase substrate, a key substrate of protein kinase C and an essential regulator of the actin cytoskeleton, cell motility, and signal transduction. MARCKS is a prominent peripheral membrane protein that cycles between membrane-bound and cytosolic states depending on phosphorylation and binding to calcium-calmodulin. The MARCKS antibody is used extensively in studies of cellular signaling, cytoskeletal rearrangement, and neuronal plasticity, providing insight into mechanisms that link phosphorylation signaling to membrane-cytoskeleton interactions.\u003cbr\u003e\u003cbr\u003eMARCKS is encoded by the MARCKS gene located on human chromosome 6q22.2. The protein is approximately 32 kilodaltons in size and is characterized by a highly basic effector domain that mediates binding to actin filaments, calmodulin, and acidic phospholipids such as phosphatidylinositol 4,5-bisphosphate (PIP2). N-terminal myristoylation allows MARCKS to anchor reversibly to the plasma membrane. Activation of protein kinase C induces phosphorylation of specific serine residues within the effector domain, causing detachment from the membrane and release of bound PIP2, thus modulating downstream calcium signaling and actin polymerization.\u003cbr\u003e\u003cbr\u003eUsing the MARCKS antibody, researchers can detect phosphorylated and non-phosphorylated forms of the protein by western blot and immunofluorescence. Western blot typically reveals a single band around 32-35 kilodaltons, while phospho-specific variants of MARCKS migrate slower due to phosphorylation-induced charge differences. Immunostaining shows cortical and membrane localization in unphosphorylated states, transitioning to a diffuse cytoplasmic distribution following stimulation with phorbol esters or calcium ionophores. MARCKS plays critical roles in cell migration, exocytosis, phagocytosis, and neuronal growth cone extension, making it a widely studied effector of signal-dependent cytoskeletal remodeling.\u003cbr\u003e\u003cbr\u003eIn the nervous system, MARCKS regulates dendritic spine morphology, synaptic vesicle trafficking, and axonal guidance. It is enriched in developing neurons and contributes to long-term potentiation and synaptic plasticity. In non-neuronal tissues, MARCKS is implicated in wound healing, immune cell chemotaxis, and cancer metastasis. Overexpression and dysregulation have been observed in glioblastoma, breast cancer, and inflammatory diseases, highlighting its function as a signaling nexus controlling cell motility and proliferation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MARCKS - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MARCKS - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MARCKS - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MARCKS - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MARCKS+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290233197,"sku":"FY12479","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9b6911aa-8e74-4ba4-9d57-e595caace7a5.jpg?v=1782237016","url":"https:\/\/www.ebiohippo.com\/products\/marcks-antibody-myristoylated-alanine-rich-c-kinase-substrate-bha17135382","provider":"BioHippo","version":"1.0","type":"link"}