{"product_id":"matk-antibody-megakaryocyte-associated-tyrosine-kinase-bha17136270","title":"MATK Antibody \/ Megakaryocyte-associated tyrosine kinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMATK Antibody \/ Megakaryocyte-associated tyrosine kinase is a anti-MATK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MATK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMATK antibody detects Megakaryocyte-associated tyrosine kinase, a cytoplasmic kinase encoded by the MATK gene located on chromosome 19q13.3. MATK belongs to the Csk (C-terminal Src kinase) family of non-receptor tyrosine kinases, acting primarily as a negative regulator of Src family kinases such as LYN, FYN, and HCK. It is expressed in hematopoietic cells, especially platelets, T cells, B cells, and megakaryocytes, where it modulates signaling cascades that control cell activation, proliferation, and differentiation. MATK serves as a key regulator of immune cell signaling and platelet function by maintaining balanced tyrosine phosphorylation levels.\u003cbr\u003e\u003cbr\u003eStructurally, MATK contains an SH3 domain, an SH2 domain, and a C-terminal catalytic kinase domain, similar to Csk, but it lacks the N-terminal myristoylation signal that targets Csk to membranes. This structural difference restricts MATK mainly to the cytoplasm, where it regulates Src kinases indirectly through adaptor-mediated interactions. MATK belongs to the protein tyrosine kinase family and shares homology with other signaling regulators such as CSK and CHK. Known interacting partners include PAG1, LAT, and LYN, which facilitate recruitment to signaling complexes.\u003cbr\u003e\u003cbr\u003eFunctionally, MATK phosphorylates inhibitory tyrosine residues within Src family kinases, leading to their inactivation and termination of receptor signaling. In platelets, MATK regulates activation by suppressing FYN and LYN kinase signaling downstream of GPVI and integrins. In immune cells, it modulates T-cell receptor (TCR) and B-cell receptor (BCR) signaling, helping to prevent overactivation and maintain immune tolerance. MATK also participates in neuronal and endothelial cell signaling, contributing to cytoskeletal organization and vascular homeostasis.\u003cbr\u003e\u003cbr\u003eMATK plays an important role in preventing hyperactivation of immune responses and maintaining hemostatic balance. Dysregulation of MATK expression or activity has been associated with autoimmune disorders, inflammatory diseases, and malignancies. Reduced MATK expression can result in excessive Src kinase activity, contributing to aberrant cell growth and survival. Conversely, overexpression has been observed in certain leukemias, where it suppresses proliferation signaling. Pathway involvement includes Src kinase signaling, platelet activation, and immune receptor regulation.\u003cbr\u003e\u003cbr\u003eThe MATK antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MATK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MATK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MATK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MATK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MATK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323197805,"sku":"FY13368","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3e3ecb8a-2d2d-40dd-9a78-b7349f8c098b.jpg?v=1782237116","url":"https:\/\/www.ebiohippo.com\/products\/matk-antibody-megakaryocyte-associated-tyrosine-kinase-bha17136270","provider":"BioHippo","version":"1.0","type":"link"}