{"product_id":"max-plasmid-purification-kit-bht15200003","title":"Max Plasmid Purification Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePlasmid Purification Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit uses a unique buffer system that combines with traditional isopropanol precipitation to quickly obtain large amounts of high-purity plasmid DNA. The plasmid DNA extracted using this kit can be applied to various routine operations, including restriction enzyme digestion, PCR, sequencing, ligation, transformation and transfection of a variety of cell experiments.\u003c\/p\u003e\n\u003cp\u003eBacterial cells are collected by centrifugation, lysed with 5 mL each of Solutions A, B and C, and the lysate is cleared through Filter column S. Plasmid DNA is precipitated with two thirds volume of isopropanol, washed twice with 6 mL of 75% ethanol and dissolved in 0.3–1 mL of Elution Buffer. From high-copy plasmids, 100 mL of culture gives about 200–1,500 µg; from low-copy plasmids, 200 mL of culture gives about 100–400 µg. Purified DNA has an OD260\/OD280 of usually about 1.8–2.0.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eFilter column with isopropanol precipitation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003eBacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input, high-copy plasmid\u003c\/th\u003e\n\u003ctd\u003e100 mL bacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input, low-copy plasmid\u003c\/th\u003e\n\u003ctd\u003e200 mL bacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eYield, high-copy plasmid\u003c\/th\u003e\n\u003ctd\u003eAbout 200–1,500 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eYield, low-copy plasmid\u003c\/th\u003e\n\u003ctd\u003eAbout 100–400 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003eOD260\/OD280 is usually about 1.8–2.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e0.3–1 mL Elution Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e10 T\u003c\/th\u003e\n\u003cth\u003e20 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNaseA\u003c\/td\u003e\n\u003ctd\u003e250 µL\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution A\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution B\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution C\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagPurp\u003c\/td\u003e\n\u003ctd\u003e250 µL\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFilter column S\u003c\/td\u003e\n\u003ctd\u003e10 vial\u003c\/td\u003e\n\u003ctd\u003e20 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRestriction enzyme digestion\u003c\/li\u003e\n\u003cli\u003ePCR\u003c\/li\u003e\n\u003cli\u003eSequencing\u003c\/li\u003e\n\u003cli\u003eLigation and transformation\u003c\/li\u003e\n\u003cli\u003eTransfection of cultured cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eThe kit is stored at room temperature (15–25°C) for 12 months, or at 2–8°C for longer storage. RNase A is stored at -20°C for 1 year; Solutions A, B and C, MagPurp, Elution Buffer and the filter columns are stored at room temperature for 1 year. Solution A is stored at 4°C once RNase A has been added to it.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHandle Solutions B and C carefully.\u003c\/li\u003e\n\u003cli\u003eAvoid violent shaking during lysis; invert the tube gently 6–8 times.\u003c\/li\u003e\n\u003cli\u003eAdd RNase A to Solution A before use and store Solution A at 4°C thereafter.\u003c\/li\u003e\n\u003cli\u003ePreheat the Elution Buffer to 65–70°C before dissolving the DNA.\u003c\/li\u003e\n\u003cli\u003eAir-dry the pellet for 10–20 min at room temperature after the ethanol washes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP003\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200003\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"10 T","offer_id":53965511786861,"sku":"EP003-10T","price":130.0,"currency_code":"USD","in_stock":true},{"title":"20 T","offer_id":53965514113389,"sku":"EP003-20T","price":234.0,"currency_code":"USD","in_stock":true},{"title":"10 T x 10","offer_id":53965514146157,"sku":"EP003-10TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"20 T x 10","offer_id":53965514178925,"sku":"EP003-20TX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP003-a-1.jpg?v=1789419187","url":"https:\/\/www.ebiohippo.com\/products\/max-plasmid-purification-kit-bht15200003","provider":"BioHippo","version":"1.0","type":"link"}