{"product_id":"mbd4-antibody-methyl-cpg-binding-domain-protein-4-bha17135388","title":"Mbd4 Antibody \/ Methyl-CpG-binding domain protein 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMbd4 Antibody \/ Methyl-CpG-binding domain protein 4 is a anti-MBD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MBD4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMbd4 antibody detects Methyl-CpG-binding domain protein 4, a nuclear enzyme that couples DNA repair with epigenetic regulation by recognizing methylated DNA and mediating the excision of mismatched bases. Mbd4 functions as a bifunctional DNA glycosylase that specifically removes thymine or uracil mispaired with guanine within methylated CpG sites, thereby maintaining genomic stability and preventing mutation accumulation. The Mbd4 antibody is widely used in studies of DNA repair, epigenetics, and cancer biology to explore the connection between cytosine methylation and genome maintenance.\u003cbr\u003e\u003cbr\u003eMbd4 is encoded by the MBD4 gene located on human chromosome 3q21.3. The protein contains two main functional domains: an N-terminal methyl-CpG-binding domain (MBD) that targets methylated DNA, and a C-terminal glycosylase domain that catalyzes base excision. Through these domains, Mbd4 directly interacts with DNA methyltransferases and participates in active DNA demethylation pathways. It also forms complexes with MLH1, a mismatch repair factor, integrating base excision repair with the mismatch repair system.\u003cbr\u003e\u003cbr\u003eThe Mbd4 antibody typically detects a 63 kilodalton protein on western blot. Mbd4 is highly expressed in proliferative tissues and cells undergoing DNA replication. Functionally, loss of Mbd4 leads to increased mutation frequency at CpG sites, predisposing cells to carcinogenesis. Experimental models show that Mbd4-deficient mice accumulate G:T mismatches and are more susceptible to DNA damage induced by oxidative stress or alkylating agents.\u003cbr\u003e\u003cbr\u003eBeyond repair, Mbd4 plays an important role in apoptosis and gene silencing. It interacts with the tumor suppressor p53 and participates in transcriptional repression of methylated gene promoters. Dysregulation of Mbd4 expression or mutations in its glycosylase domain have been linked to colorectal cancer, glioma, and leukemia, where defective base excision repair contributes to genomic instability.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MBD4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MBD4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MBD4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MBD4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MBD4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290921325,"sku":"FY12485","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0182cb37-363a-4088-8c3d-7857f76bf7f0.jpg?v=1782237017","url":"https:\/\/www.ebiohippo.com\/products\/mbd4-antibody-methyl-cpg-binding-domain-protein-4-bha17135388","provider":"BioHippo","version":"1.0","type":"link"}