{"product_id":"mcherry-bhn20152551","title":"mCherry","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis GenCefe circular RNA (circRNA) encodes \u003cstrong\u003emCherry\u003c\/strong\u003e, a reporter gene construct supplied for circular RNA delivery tracing and expression tracking. The product is formulated as lyophilised, non-encapsulated circular RNA and is intended for use in cell-based research applications requiring circular RNA stability.\u003c\/p\u003e\n\u003ch2\u003ecircRNA Construct Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eRNA Architecture:\u003c\/strong\u003e Circular RNA — no free 5′ or 3′ termini; resistant to cellular 5′–3′ and 3′–5′ exonucleases, conferring inherently greater intracellular stability than linear mRNA.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eTranslation Mechanism:\u003c\/strong\u003e Cap-independent; relies on internal ribosome entry site (IRES) or rolling-circle translation depending on construct design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSpecies:\u003c\/strong\u003e Human\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilised powder; reconstitute in DEPC-treated water as needed.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA Length:\u003c\/strong\u003e Provided upon order placement.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSupplied as non-encapsulated circRNA; formulation selection (LNP, electroporation, lipofection) is at the discretion of the end user.\u003c\/p\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eReporter proteins are widely used in molecular and cell biology as observable readouts for gene expression, mRNA delivery efficiency, and intracellular trafficking. Fluorescent proteins such as EGFP, mCherry, and their derivatives emit detectable signals under specific excitation wavelengths, enabling real-time live-cell imaging and quantitative flow cytometry. Bioluminescent reporters, including Firefly luciferase (Fluc) and \u003cem\u003eRenilla\u003c\/em\u003e luciferase, catalyse light-producing reactions that allow highly sensitive detection in plate-based assays with minimal background. Reporter mRNAs are particularly valuable for benchmarking lipid nanoparticle (LNP) formulations, electroporation parameters, and transfection reagents before transitioning to therapeutically relevant cargo.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003emRNA delivery benchmarking: Reporter mRNAs are used as surrogate payloads to rapidly optimise LNP formulations, electroporation parameters, and transfection reagent doses before committing costly therapeutic cargo to delivery trials.\u003c\/li\u003e\n  \u003cli\u003eOrthogonal dual-reporter assays: EGFP\/mCherry + Firefly\/Renilla dual-reporter mRNA pairs allow internal normalisation of delivery efficiency versus transcriptional output in the same cell population.\u003c\/li\u003e\n  \u003cli\u003eCircular RNA reporters: circRNA-based reporter constructs (e.g., circEGFP, circFluc) are being explored as longer-lived, cap-independent expression platforms compared to conventional linear mRNA, with resistance to cellular 5′–3′ exonucleases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003emRNA delivery efficiency benchmarking — measuring fluorescence or bioluminescence as surrogate readout for payload delivery and expression kinetics across formulations.\u003c\/li\u003e\n  \u003cli\u003eTransfection optimisation — titrating reagent dose, mRNA concentration, and time-points using rapidly detectable reporter signals before transitioning to functional payloads.\u003c\/li\u003e\n  \u003cli\u003eIntracellular tracking — live-cell imaging with fluorescent reporter mRNAs to monitor subcellular localisation and translation dynamics in real time.\u003c\/li\u003e\n  \u003cli\u003eDual-reporter normalisation — paired reporter constructs (e.g., EGFP-Fluc, mCherry-Renilla) provide internal controls for delivery efficiency versus expression output.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eReporter signal intensity varies with transfection efficiency, cell health, and mRNA dose; include an untransfected negative control and a parallel positive control at all time-points.\u003c\/li\u003e\n  \u003cli\u003eFluorescent reporter mRNAs (EGFP, mCherry) express within 4–6 h post-transfection; bioluminescent reporters (Fluc, Renilla) typically reach peak signal 12–24 h post-transfection depending on substrate availability.\u003c\/li\u003e\n  \u003cli\u003ePA4T- and PA-tagged variants include additional protein sequences that may affect fluorescence intensity or antibody detection; confirm variant compatibility with downstream detection methods before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"GenCefe Biotech","offers":[{"title":"20 ug","offer_id":53239634133357,"sku":"IR0106002","price":398.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53239650976109,"sku":"IR0106005","price":644.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53239651008877,"sku":"IR0106100","price":3149.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/circRNA_ba2b2ce5-7073-421e-b1de-dc2715169392.png?v=1776677731","url":"https:\/\/www.ebiohippo.com\/products\/mcherry-bhn20152551","provider":"BioHippo","version":"1.0","type":"link"}