{"product_id":"mcherry-pa4t-bhn20152454","title":"mCherry-PA4T","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis GenCefe mRNA encodes \u003cstrong\u003emCherry-PA4T\u003c\/strong\u003e, a reporter gene construct supplied for mRNA delivery tracing and transfection efficiency studies. The product is formulated as lyophilised, non-encapsulated RNA and is intended for use in cell-based research applications requiring transient protein expression with reduced immunogenicity.\u003c\/p\u003e\n\u003ch2\u003emRNA Construct Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ Cap:\u003c\/strong\u003e Cap1 (m7GpppNm) — co-transcriptionally added during in vitro transcription (IVT). Cap1 includes 2′-O-methylation at the first transcribed nucleotide, closely mimicking the cap structure found on endogenous mammalian mRNA and reducing recognition by innate immune sensors (e.g., IFIT1\/IFIT3).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eModified Nucleotides:\u003c\/strong\u003e 100% N1-methylpseudouridine (m1Ψ; N1-Me-Pseudo UTP) substitution for all uridine residues. m1Ψ modification reduces TLR7\/TLR8-mediated innate immune activation and PKR-driven translational suppression, resulting in improved protein expression in immunocompetent cells and primary cell types.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePoly(A) Tail:\u003c\/strong\u003e 100–120 nt — enzymatically polyadenylated. The poly(A) tail stabilises the 3′ terminus, supports poly(A)-binding protein (PABP) recruitment, and enhances ribosome recycling for efficient cap-dependent translation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 5′ UTR) — a well-characterised human UTR that supports efficient cap-dependent translation initiation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e3′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 3′ UTR) — provides post-transcriptional stability and modulates mRNA decay kinetics.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSignal Peptide:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein Tag:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCodon Optimisation:\u003c\/strong\u003e No (native human codon usage retained)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA Length:\u003c\/strong\u003e Provided upon order placement.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilised powder; reconstitute in DEPC-treated water as needed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis mRNA is supplied as non-encapsulated, lyophilised powder. Delivery vehicle selection (LNP, electroporation, lipofection) is at the discretion of the end user and should be optimised for the target cell type and application.\u003c\/p\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eReporter proteins are widely used in molecular and cell biology as observable readouts for gene expression, mRNA delivery efficiency, and intracellular trafficking. Fluorescent proteins such as EGFP, mCherry, and their derivatives emit detectable signals under specific excitation wavelengths, enabling real-time live-cell imaging and quantitative flow cytometry. Bioluminescent reporters, including Firefly luciferase (Fluc) and \u003cem\u003eRenilla\u003c\/em\u003e luciferase, catalyse light-producing reactions that allow highly sensitive detection in plate-based assays with minimal background. Reporter mRNAs are particularly valuable for benchmarking lipid nanoparticle (LNP) formulations, electroporation parameters, and transfection reagents before transitioning to therapeutically relevant cargo.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003emRNA delivery benchmarking: Reporter mRNAs are used as surrogate payloads to rapidly optimise LNP formulations, electroporation parameters, and transfection reagent doses before committing costly therapeutic cargo to delivery trials.\u003c\/li\u003e\n  \u003cli\u003eOrthogonal dual-reporter assays: EGFP\/mCherry + Firefly\/Renilla dual-reporter mRNA pairs allow internal normalisation of delivery efficiency versus transcriptional output in the same cell population.\u003c\/li\u003e\n  \u003cli\u003eCircular RNA reporters: circRNA-based reporter constructs (e.g., circEGFP, circFluc) are being explored as longer-lived, cap-independent expression platforms compared to conventional linear mRNA, with resistance to cellular 5′–3′ exonucleases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003emRNA delivery efficiency benchmarking — measuring fluorescence or bioluminescence as surrogate readout for payload delivery and expression kinetics across formulations.\u003c\/li\u003e\n  \u003cli\u003eTransfection optimisation — titrating reagent dose, mRNA concentration, and time-points using rapidly detectable reporter signals before transitioning to functional payloads.\u003c\/li\u003e\n  \u003cli\u003eIntracellular tracking — live-cell imaging with fluorescent reporter mRNAs to monitor subcellular localisation and translation dynamics in real time.\u003c\/li\u003e\n  \u003cli\u003eDual-reporter normalisation — paired reporter constructs (e.g., EGFP-Fluc, mCherry-Renilla) provide internal controls for delivery efficiency versus expression output.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eReporter signal intensity varies with transfection efficiency, cell health, and mRNA dose; include an untransfected negative control and a parallel positive control at all time-points.\u003c\/li\u003e\n  \u003cli\u003eFluorescent reporter mRNAs (EGFP, mCherry) express within 4–6 h post-transfection; bioluminescent reporters (Fluc, Renilla) typically reach peak signal 12–24 h post-transfection depending on substrate availability.\u003c\/li\u003e\n  \u003cli\u003ePA4T- and PA-tagged variants include additional protein sequences that may affect fluorescence intensity or antibody detection; confirm variant compatibility with downstream detection methods before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"GenCefe Biotech","offers":[{"title":"20 ug","offer_id":53239632822637,"sku":"IR0009002","price":279.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53239642030445,"sku":"IR0009010","price":339.0,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53239642063213,"sku":"IR0009020","price":399.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53239642095981,"sku":"IR0009050","price":459.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53239642128749,"sku":"IR0009100","price":549.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":53239642161517,"sku":"IR0009500","price":1799.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mRNA_bdeaa9d8-5570-4932-b45d-e37551e78211.png?v=1776677726","url":"https:\/\/www.ebiohippo.com\/products\/mcherry-pa4t-bhn20152454","provider":"BioHippo","version":"1.0","type":"link"}