{"product_id":"mcm9-antibody-minichromosome-maintenance-complex-component-9-bha17135828","title":"MCM9 Antibody \/ Minichromosome maintenance complex component 9","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMCM9 Antibody \/ Minichromosome maintenance complex component 9 is a anti-MCM9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MCM9\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMCM9 antibody detects Minichromosome maintenance complex component 9, a helicase-associated protein essential for DNA replication licensing and homologous recombination repair. Encoded by the MCM9 gene on chromosome 6q22.31, this nuclear protein functions as part of the MCM8-MCM9 complex, which assists in DNA replication origin activation and double-strand break repair. MCM9 plays a critical role in maintaining genomic stability and facilitating proper DNA synthesis during S phase.\u003cbr\u003e\u003cbr\u003eStructurally, MCM9 is a 1,143-amino-acid nuclear protein of approximately 130 kilodaltons containing an AAA+ ATPase domain typical of MCM family helicases. Although it lacks intrinsic helicase activity, MCM9 interacts with MCM8 to form a functional complex that promotes DNA end resection and strand invasion during homologous recombination. It also associates with replication proteins such as RPA and RAD51, coordinating DNA repair with replication fork progression.\u003cbr\u003e\u003cbr\u003eThe MCM9 antibody is widely used in cell cycle, DNA repair, and cancer research to study replication licensing, recombination mechanisms, and genome maintenance. Western blot analysis detects a 130 kilodalton band corresponding to MCM9, while immunofluorescence shows distinct nuclear foci that colocalize with replication and repair markers. This antibody enables detailed analysis of DNA replication and damage response pathways.\u003cbr\u003e\u003cbr\u003eFunctionally, MCM9 is required for the recruitment of repair proteins to sites of DNA damage and for the restart of stalled replication forks. Mutations in MCM9 are associated with hereditary colorectal cancer syndromes and premature ovarian failure due to impaired DNA repair capacity. Loss of MCM9 function results in genomic instability and sensitivity to DNA-damaging agents. The MCM9 antibody provides a key reagent for investigating replication integrity, homologous recombination, and tumor suppression mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MCM9 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MCM9 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MCM9 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MCM9 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MCM9+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306977645,"sku":"FY12926","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8224fc59-4de9-4b7a-a762-428197354318.jpg?v=1782237066","url":"https:\/\/www.ebiohippo.com\/products\/mcm9-antibody-minichromosome-maintenance-complex-component-9-bha17135828","provider":"BioHippo","version":"1.0","type":"link"}