{"product_id":"mcmbp-antibody-mcm-binding-protein-bha17135387","title":"MCMBP Antibody \/ MCM-binding protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMCMBP Antibody \/ MCM-binding protein is a anti-MCMBP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MCMBP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMCMBP antibody detects Minichromosome maintenance complex-binding protein, a nuclear protein that regulates DNA replication and cell cycle progression. MCMBP interacts with the MCM (minichromosome maintenance) helicase complex, ensuring its proper assembly and nuclear localization. It functions as a chaperone stabilizing MCM subunits before DNA replication initiation. The MCMBP antibody is valuable in studies of DNA replication licensing, genome stability, and cell proliferation control.\u003cbr\u003e\u003cbr\u003eMCMBP is encoded by the MCMBP gene located on human chromosome 10q24.2. The protein is approximately 70 kilodaltons in size and associates with MCM subunits MCM2-MCM7. These interactions facilitate the transport of MCM complexes into the nucleus and their incorporation into pre-replication complexes at replication origins. MCMBP assists in maintaining replication fork integrity and prevents unscheduled replication events that could lead to genomic instability. It also contributes to the disassembly of replication machinery during mitotic exit.\u003cbr\u003e\u003cbr\u003eThe MCMBP antibody identifies the 70 kilodalton protein by western blot and reveals nuclear localization during S phase, with partial cytoplasmic presence in early G1. Immunofluorescence shows co-localization with replication foci, supporting its role in DNA synthesis regulation. Functional studies demonstrate that depletion of MCMBP causes replication stress, reduced origin firing, and accumulation of DNA damage markers such as gamma-H2AX. MCMBP interacts with replication factor C and proliferating cell nuclear antigen, integrating replication initiation with elongation control.\u003cbr\u003e\u003cbr\u003eBeyond DNA replication, MCMBP is involved in maintaining chromosomal stability and has been linked to mitotic checkpoint regulation. Overexpression has been reported in certain cancers, including hepatocellular and colorectal carcinomas, where it may promote cell cycle progression. Conversely, its loss sensitizes cells to replication inhibitors, suggesting potential as a therapeutic target.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MCMBP - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MCMBP - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MCMBP - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MCMBP - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MCMBP+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290691949,"sku":"FY12484","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_28f8f989-5e19-4335-b0d2-34d286d21040.jpg?v=1782237012","url":"https:\/\/www.ebiohippo.com\/products\/mcmbp-antibody-mcm-binding-protein-bha17135387","provider":"BioHippo","version":"1.0","type":"link"}