{"product_id":"mecp2-antibody-methyl-cpg-binding-protein-2-bha17136167","title":"MECP2 Antibody \/ Methyl-CpG-binding protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMECP2 Antibody \/ Methyl-CpG-binding protein 2 is a anti-MECP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Monkey, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MECP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMECP2 antibody detects Methyl-CpG-binding protein 2, a nuclear chromatin-associated protein that binds methylated DNA and regulates gene expression. The UniProt recommended name is Methyl-CpG-binding protein 2 (MECP2). This transcriptional regulator modulates chromatin compaction and transcriptional repression through its interactions with methylated CpG sites and chromatin remodeling complexes.\u003cbr\u003e\u003cbr\u003eFunctionally, MECP2 antibody identifies a 486-amino-acid protein composed of a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD). MECP2 binds selectively to methylated DNA sequences and recruits co-repressors such as SIN3A and histone deacetylases (HDACs), leading to histone deacetylation and chromatin condensation. It also regulates non-coding RNA transcription, alternative splicing, and higher-order chromatin organization. MECP2 plays a critical role in neuronal maturation, synaptic development, and activity-dependent gene regulation.\u003cbr\u003e\u003cbr\u003eThe MECP2 gene is located on chromosome Xq28 and is expressed ubiquitously, but at especially high levels in neurons. Expression is dynamically regulated during postnatal brain development, paralleling synaptogenesis. In neurons, MECP2 controls transcriptional homeostasis by balancing activation and repression of large gene networks essential for synaptic plasticity and neuronal connectivity.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in MECP2 cause Rett syndrome, a severe neurodevelopmental disorder characterized by regression of motor and cognitive abilities in early childhood. Different mutation types (missense, nonsense, deletions) disrupt DNA binding or repressor recruitment, leading to widespread transcriptional dysregulation. Altered MECP2 expression is also implicated in autism spectrum disorder and certain cancers. Research using MECP2 antibody supports studies in epigenetic regulation, neurodevelopment, and chromatin biology.\u003cbr\u003e\u003cbr\u003eMECP2 antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect methyl-DNA-binding proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Methyl-CpG-binding protein 2 contains an MBD that recognizes methylated CpG dinucleotides, a TRD that interacts with SIN3A-HDAC complexes, and a C-terminal domain that binds nucleosomes. This structural modularity allows MECP2 to act as a chromatin architectural protein coordinating DNA methylation and transcriptional silencing. This antibody enables detailed analysis of MECP2's role in gene regulation and neurological disease mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MECP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MECP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MECP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MECP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MECP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321264493,"sku":"FY13265","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7156741b-8c79-46cb-8f15-da8e0c349a8e.jpg?v=1782237078","url":"https:\/\/www.ebiohippo.com\/products\/mecp2-antibody-methyl-cpg-binding-protein-2-bha17136167","provider":"BioHippo","version":"1.0","type":"link"}