{"product_id":"med4-antibody-biotin-conjugated-bha10528139","title":"MED4 Antibody, Biotin conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-MED4 antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect MED4 in Human for others research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human Mediator of RNA polymerase II transcription subunit 4 protein (167-270AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiotin:\u003c\/strong\u003e Streptavidin\/avidin amplification; block endogenous biotin in biotin-rich samples.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eMED4\u003c\/strong\u003e (also: Activator-recruited cofactor 36 kDa component, ARC36, DRIP36, FLJ10956, HSPC126, med4, MED4_HUMAN, Mediator complex subunit 4, Mediator of RNA polymerase II transcription subunit 4, Mediator of RNA polymerase II transcription, subunit 4 homolog, OTTHUMP00000018390, OTTHUMP00000018391, OTTHUMP00000018392, RP11-90M2.2, TRAP\/SMCC\/PC2 subunit p36 subunit, TRAP36, VDRIP, Vitamin D3 receptor-interacting protein complex 36 kDa component) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current MED4 annotation relevant to others.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53240814272877,"sku":"CSB-PA865098LD01HU-50UG","price":166.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53240916967789,"sku":"CSB-PA865098LD01HU-100UG","price":299.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/med4-antibody-biotin-conjugated-bha10528139","provider":"BioHippo","version":"1.0","type":"link"}