{"product_id":"med7-antibody-mediator-of-rna-polymerase-ii-transcription-subunit-7-bha17136331","title":"MED7 Antibody \/ Mediator of RNA polymerase II transcription subunit 7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMED7 Antibody \/ Mediator of RNA polymerase II transcription subunit 7 is a anti-MED7 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleolus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MED7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IF, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMED7 (Mediator of RNA polymerase II transcription subunit 7) is a component of the Mediator complex, a multiprotein assembly that serves as a bridge between transcription factors and RNA polymerase II. MED7 plays a role in regulating transcription initiation, elongation, and co-activation of gene expression across diverse cellular processes.    \u003cp\u003eMED7 is ubiquitously expressed and contributes to the regulation of genes involved in development, cell cycle progression, and differentiation. Dysregulation of Mediator subunits, including MED7, has been linked to transcriptional misregulation in cancer and developmental disorders, making it an important target in transcriptional biology research. \u003c\/p\u003e\n\u003cp\u003eUsing a high-quality MED7 antibody allows sensitive detection in applications such as western blot, immunohistochemistry, and chromatin immunoprecipitation. A MED7 antibody from\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MED7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MED7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MED7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MED7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MED7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047325524333,"sku":"RQ8912","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b3a250ec-346b-490e-b9ce-be2cc40d392e.jpg?v=1772019445","url":"https:\/\/www.ebiohippo.com\/products\/med7-antibody-mediator-of-rna-polymerase-ii-transcription-subunit-7-bha17136331","provider":"BioHippo","version":"1.0","type":"link"}